The Arabidopsis SKP1-LIKE1 gene is essential for male meiosis and may control homologue separation

Yang, M., Hu, Y., Lodhi, M., McCombie, W. R., Ma, H. (1999) The Arabidopsis SKP1-LIKE1 gene is essential for male meiosis and may control homologue separation. Proceedings of the National Academy of Sciences of the United States of America, 96 (20). pp. 11416-11421. ISSN 00278424 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/10500191
DOI: 10.1073/pnas.96.20.11416

Abstract

The yeast and human SKP1 genes regulate the mitotic cell cycle but are not yet known to be required for meiosis. Nine Arabidopsis SKP1 homologues have been uncovered and are named ASK1 through ASK9. Here, we report the isolation and characterization of a male sterile Arabidopsis mutant and show that the mutant defect was caused by a Ds transposon insertion into the ASK1 gene. In the ask1-1 mutant, abnormal microspores exhibit a range of sizes. Furthermore, during mutant male meiosis, although homologous chromosome pairing appeared normal at metaphase I, chromosome segregation at anaphase I is unequal, and some chromosomes are abnormally extended. Therefore, in ask1- 1, at least some homologues remain associated after metaphase I. In addition, immunofluorescence microscopy indicates that the mutant spindle morphology at both metaphase I and early anaphase I is normal; thus, the abnormal chromosome segregation is not likely caused by a spindle defect. Because the yeast Skp1p is required for targeting specific proteins for ubiquitin- mediated proteolysis, we propose that ASK1 controls homologue separation by degrading or otherwise removing a protein that is required directly or indirectly for homologue association before anaphase I.

Item Type: Paper
Uncontrolled Keywords: ubiquitin vegetable protein arabidopsis article cell cycle chromosome pairing chromosome segregation controlled study gene gene insertion gene isolation gene mutation genetic analysis male meiosis microsporida mitosis nonhuman priority journal protein degradation transposon Amino Acid Sequence Arabidopsis Proteins Base Sequence Cell Cycle Proteins Chromosomes Genes, Plant Molecular Sequence Data Mutation Plant Proteins S-Phase Kinase-Associated Proteins Embryophyta Microsporidia
Subjects: organism description > plant > Arabidopsis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
organs, tissues, organelles, cell types and functions > organelles, types and functions > meiosis
organism description > plant
CSHL Authors:
Communities: CSHL labs > McCombie lab
Depositing User: Matt Covey
Date Deposited: 25 Apr 2013 20:16
Last Modified: 25 Apr 2013 20:16
PMCID: PMC18048
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28193

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