Novel anchorage of GluR2/3 to the postsynaptic density by the AMPA receptor-binding protein ABP

Srivastava, S., Osten, P., Vilim, F. S., Khatri, L., Inman, G., States, B., Daly, C., DeSouza, S., Abagyan, R., Valtschanoff, J. G., Weinberg, R. J., Ziff, E. B. (1998) Novel anchorage of GluR2/3 to the postsynaptic density by the AMPA receptor-binding protein ABP. Neuron, 21 (3). pp. 581-591. ISSN 08966273 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/9768844
DOI: 10.1016/s0896-6273(00)80568-1

Abstract

We report the cloning of α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptor-binding protein (ABP), a postsynaptic density (PSD) protein related to glutamate receptor-interacting protein (GRIP) with two sets of three PDZ domains, which binds the GluR2/3 AMPA receptor subunits. ABP exhibits widespread CNS expression and is found at the postsynaptic membrane. We show that the protein interactions of the ABP/GRIP family differ from the PSD-95 family, which binds N-methyl-D-aspartate (NMDA) receptors. ABP binds to the GluR2/3 C-terminal VKI-COOH motif via class II hydrophobic PDZ interactions, distinct from the class I PSD-95-NMDA receptor interaction. ABP and GRIP also form homo- and heteromultimers through PDZ- PDZ interactions but do not bind PSD-95. We suggest that the ABP/GRIP and PSD-95 families form distinct scaffolds that anchor, respectively, AMPA and NMDA receptors.

Item Type: Paper
Uncontrolled Keywords: AMPA receptor binding protein glutamate receptor n methyl dextro aspartic acid receptor animal tissue article hydrophobicity male molecular cloning nonhuman postsynaptic membrane priority journal protein binding protein expression protein protein interaction rat receptor binding Amino Acid Sequence Animals Binding Sites Brain Carrier Proteins Cloning, Molecular Gene Expression Regulation, Developmental Macromolecular Substances Models, Molecular Molecular Sequence Data Nerve Tissue Proteins Peptide Fragments Protein Conformation Rats Receptors, AMPA Recombinant Proteins Sequence Alignment Spinal Cord Synapses Transcription, Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > AMPA receptor
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
organs, tissues, organelles, cell types and functions > organs types and functions > brain
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > glutamate receptor
organs, tissues, organelles, cell types and functions > organs types and functions
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
CSHL Authors:
Communities: CSHL labs > Osten lab
Depositing User: Matt Covey
Date: 1998
Date Deposited: 04 Apr 2013 21:15
Last Modified: 04 Apr 2013 21:15
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28095

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