Evidence for a new, high-molecular weight isoform of protein kinase C in rat hippocampus

Sublette, E., Naik, M. U., Jiang, X., Osten, P., Valsamis, H., Osada, S., Ohno, S., Sacktor, T. C. (1993) Evidence for a new, high-molecular weight isoform of protein kinase C in rat hippocampus. Neuroscience Letters, 159 (1-2). pp. 175-178. ISSN 03043940 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/8264963

Abstract

We describe a new form of protein kinase C (PKC) with a molecular weight of 97 kDa, higher than the known forms of vertebrate PKC. This putative new high-molecular weight isoform, which we are calling PKC(HMW), is increased in the membrane fraction either upon application of phorbol esters or with afferent synaptic stimulation of Schaffer collaterals in hippocampal slices. The protein cross-reacts on immunoblot with affinity-purified polyclonal antiserum raised against a peptide derived from the carboxy-terminus of PKCη; it does not cross-react, however, with antiserum against the amino-terminal region of PKCη. In the tissues examined, PKC(HMW) is localized primarily in brain, in contrast to PKCη, which is found predominantly in lung and skin.

Item Type: Paper
Uncontrolled Keywords: brain long-term potentiation phorbol ester PKC PKCη isoenzyme protein kinase c adolescent animal tissue article brain slice controlled study hippocampus immunoblotting long term potentiation nonhuman priority journal rat Amino Acid Sequence Animal Isoenzymes Molecular Sequence Data Molecular Weight Neurons, Afferent Phorbol Esters Rats Support, U.S. Gov't, P.H.S.
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > Long term potentiation
organs, tissues, organelles, cell types and functions > cell types and functions > cell types
organs, tissues, organelles, cell types and functions > cell types and functions > cell types
organs, tissues, organelles, cell types and functions > cell types and functions > cell types

organs, tissues, organelles, cell types and functions > cell types and functions
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes
organs, tissues, organelles, cell types and functions > tissues types and functions > hippocampus
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
organs, tissues, organelles, cell types and functions > tissues types and functions
CSHL Authors:
Communities: CSHL labs > Osten lab
Depositing User: Matt Covey
Date Deposited: 05 Apr 2013 02:25
Last Modified: 05 Apr 2013 02:25
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28091

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