Spontaneous rhythmic field potentials of isolated mouse hippocampal-subicular-entorhinal cortices in vitro

Wu, C. P., Huang, H. L., Asl, M. N., He, J. W., Gillis, J., Skinner, F. K., Zhang, L. (2006) Spontaneous rhythmic field potentials of isolated mouse hippocampal-subicular-entorhinal cortices in vitro. Journal of Physiology, 576 (2). pp. 457-476. ISSN 00223751 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/16887877
DOI: 10.1113/jphysiol.2006.114918

Abstract

The rodent hippocampal circuit is capable of exhibiting in vitro spontaneous rhythmic field potentials (SRFPs) of 1-4 Hz that originate from the CA3 area and spread to the CA1 area. These SRFPs are largely correlated with GABA-A IPSPs in pyramidal neurons and repetitive discharges in inhibitory interneurons. As such, their generation is thought to result from cooperative network activities involving both pyramidal neurons and GABAergic interneurons. Considering that the hippocampus, subiculum and entorhinal cortex function as an integrated system crucial for memory and cognition, it is of interest to know whether similar SRFPs occur in hippocampal output structures (that is, the subiculum and entorhinal cortex), and if so, to understand the cellular basis of these subicular and entorhinal SRFPs as well as their temporal relation to hippocampal SRFPs. We explored these issues in the present study using thick hippocampal-subicular-entorhinal cortical slices prepared from adult mice. SRFPs were found to spread from the CA1 area to the subicular and entorhinal cortical areas. Subicular and entorhinal cortical SRFPs were correlated with mixed IPSPs/EPSPs in local pyramidal neurons, and their generation was dependent upon the activities of GABA-A and AMPA glutamate receptors. In addition, the isolated subicular circuit could elicit SRFPs independent of CA3 inputs. We hypothesize that the SRFPs represent a basal oscillatory activity of the hippocampal-subicular-entorhinal cortices and that the subiculum functions as both a relay and an amplifier, spreading the SRFPs from the hippocampus to the entorhinal cortex. © 2006 The Authors. Journal compilation © 2006 The Physiological Society.

Item Type: Paper
Uncontrolled Keywords: 4 aminobutyric acid A receptor AMPA receptor animal tissue article brain electrophysiology brain function controlled study correlation analysis entorhinal cortex GABAergic system hippocampus potential hippocampus theta rhythm in vitro study inhibitory postsynaptic potential interneuron mouse nerve cell membrane potential nerve cell network nonhuman oscillatory potential priority journal pyramidal nerve cell subiculum Action Potentials Animals Electroencephalography Electrophysiology Hippocampus Interneurons Mice Mice, Inbred C57BL Neurons, Afferent Patch-Clamp Techniques Periodicity Pyramidal Cells Receptors, GABA-A
Subjects: Investigative techniques and equipment
Investigative techniques and equipment > electrophysiology
organs, tissues, organelles, cell types and functions > tissues types and functions > hippocampus
organism description > animal > mammal > rodent > mouse
organs, tissues, organelles, cell types and functions > tissues types and functions
CSHL Authors:
Communities: CSHL labs > Gillis Lab
Depositing User: Matt Covey
Date Deposited: 04 Apr 2013 13:35
Last Modified: 04 Apr 2013 13:35
PMCID: PMC1890361
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28088

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