Size does matter: Generation of intrinsic network rhythms in thick mouse hippocampal slices

Wu, C., Luk, W. P., Gillis, J., Skinner, F., Zhang, L. (2005) Size does matter: Generation of intrinsic network rhythms in thick mouse hippocampal slices. Journal of Neurophysiology, 93 (4). pp. 2302-2317. ISSN 00223077 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/15537814
DOI: 10.1152/jn.00806.2004

Abstract

Rodent hippocampal slices of ≤0.5 mm thickness have been widely used as a convenient in vitro model since the 1970s. However, spontaneous population rhythmic activities do not consistently occur in this preparation due to limited network connectivity. To overcome this limitation, we develop a novel slice preparation of 1 mm thickness from adult mouse hippocampus by separating dentate gyrus from CA3/CA1 areas but preserving dentate-CA3-CA1 connectivity. While superfused in vitro at 32 or 37°C, the thick slice exhibits robust spontaneous network rhythms of 1-4 Hz that originate from the CA3 area. Via assessing tissue O2, K+, pH, synaptic, and single-cell activities of superfused thick slices, we verify that these spontaneous rhythms are not a consequence of hypoxia and nonspecific experimental artifacts. We suggest that the thick slice contains a unitary circuitry sufficient to generate intrinsic hippocampal network rhythms and this preparation is suitable for exploring the fundamental properties and plasticity of a functionally defined hippocampal "lamella" in vitro. Copyright © 2005 The American Physiological Society.

Item Type: Paper
Uncontrolled Keywords: potassium ion animal tissue article artifact brain region brain size brain slice dentate gyrus hippocampus hypoxia in vitro study mouse nerve cell network nerve cell plasticity nonhuman pH priority journal synapse tissue oxygenation Action Potentials Animals Cell Size Mice Mice, Inbred C57BL Nerve Net Organ Culture Techniques Periodicity
Subjects: Investigative techniques and equipment
Investigative techniques and equipment > electrophysiology
organs, tissues, organelles, cell types and functions > tissues types and functions > hippocampus
organs, tissues, organelles, cell types and functions > tissues types and functions
CSHL Authors:
Communities: CSHL labs > Gillis Lab
Depositing User: Matt Covey
Date Deposited: 04 Apr 2013 13:55
Last Modified: 04 Apr 2013 13:55
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28086

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