An in vitro model of hippocampal sharp waves: Regional initiation and intracellular correlates

Wu, C., Asl, M. N., Gillis, J., Skinner, F. K., Zhang, L. (2005) An in vitro model of hippocampal sharp waves: Regional initiation and intracellular correlates. Journal of Neurophysiology, 94 (1). pp. 741-753. ISSN 00223077 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/15772241
DOI: 10.1152/jn.00086.2005

Abstract

During slow wave sleep and consummatory behaviors, electroencephalographic recordings from the rodent hippocampus reveal large amplitude potentials called sharp waves. The sharp waves originate from the CA3 circuitry and their generation is correlated with coherent discharges of CA3 pyramidal neurons and dependent on activities mediated by AMPA glutamate receptors. To model sharp waves in a relatively large hippocampal circuitry in vitro, we developed thick (1 mm) mouse hippocampal slices by separating the dentate gyrus from the CA2/CA1 areas while keeping the functional dentate gyrus-CA3-CA1 connections. We found that large amplitude (0.3-3 mV) sharp wave-like field potentials occurred spontaneously in the thick slices without extra ionic or pharmacological manipulation and they resemble closely electroencephalographic sharp waves with respect to waveform, regional initiation, pharmacological manipulations, and intracellular correlates. Through measuring tissue O2, K+, and synaptic and single cell activities, we verified that the sharp wave-like potentials are not a consequence of anoxia, nonspecific elevation of extracellular K+ and dissection-related tissue damage. Our data suggest that a subtle but crucial increase in the CA3 glutamatergic activity effectively recruits a population of neurons thus responsible for the generation of the sharp wave-like spontaneous field potentials in isolated hippocampal circuitry. Copyright © 2005 The American Physiological Society.

Item Type: Paper
Uncontrolled Keywords: AMPA receptor glutamate receptor oxygen potassium ion adolescent animal behavior animal experiment article cell activity controlled study dentate gyrus electroencephalogram electroencephalography hippocampus in vitro study model mouse nerve potential newborn nonhuman priority journal pyramidal nerve cell slow wave sleep synapse waveform Analysis of Variance Anesthetics Animals Animals, Newborn Anoxia Bicuculline Chelating Agents Dose-Response Relationship, Radiation Drug Interactions Egtazic Acid Electric Stimulation Electrodes Evoked Potentials Excitatory Amino Acid Antagonists Excitatory Postsynaptic Potentials Extracellular Space GABA Antagonists Membrane Potentials Methohexital Mice Mice, Inbred C57BL Neural Inhibition Neural Pathways Neurons Patch-Clamp Techniques Potassium Synaptic Transmission
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > AMPA receptor
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
Investigative techniques and equipment
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
Investigative techniques and equipment > electrophysiology
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > glutamate receptor
organs, tissues, organelles, cell types and functions > tissues types and functions > hippocampus
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
organs, tissues, organelles, cell types and functions > tissues types and functions
CSHL Authors:
Communities: CSHL labs > Gillis Lab
Depositing User: Matt Covey
Date Deposited: 04 Apr 2013 13:46
Last Modified: 04 Apr 2013 13:46
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28081

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