Skeletal muscle pericyte subtypes differ in their differentiation potential

Birbrair, A., Zhang, T., Wang, Z. M., Messi, M. L., Enikolopov, G. N., Mintz, A., Delbono, O. (January 2013) Skeletal muscle pericyte subtypes differ in their differentiation potential. Stem Cell Research, 10 (1). pp. 67-84. ISSN 1873-5061

URL: http://www.ncbi.nlm.nih.gov/pubmed/23128780
DOI: 10.1016/j.scr.2012.09.003

Abstract

Neural progenitor cells have been proposed as a therapy for central nervous system disorders, including neurodegenerative diseases and trauma injuries, however their accessibility is a major limitation. We recently isolated Tuj1+ cells from skeletal muscle culture of Nestin-GFP transgenic mice however whether they form functional neurons in the brain is not yet known. Additionally, their isolation from nontransgenic species and identification of their ancestors is unknown. This gap of knowledge precludes us from studying their role as a valuable alternative to neural progenitors. Here, we identified two pericyte subtypes, type-1 and type-2, using a double transgenic Nestin-GFP/NG2-DsRed mouse and demonstrated that Nestin-GFP+/Tuj1+ cells derive from type-2 Nestin-GFP+/NG2-DsRed+/CD146+ pericytes located in the skeletal muscle interstitium. These cells are bipotential as they generate either Tuj1+ cells when cultured with muscle cells or become "classical" alpha-SMA+pericytes when cultured alone. In contrast, type-1 Nestin-GFP-/NG2-DsRed+/CD146+ pericytes generate alpha-SMA+pericytes but not Tuj1+ cells. Interestingly, type-2 pericyte derived Tuj1+ cells retain some pericytic markers (CD146+/PDGFR beta+/NG2+). Given the potential application of Nestin-GFP+/NG2-DsRed+/Tuj1+ cells for cell therapy, we found a surface marker, the nerve growth factor receptor, which is expressed exclusively in these cells and can be used to identify and isolate them from mixed cell populations in nontransgenic species for clinical purposes. (C) 2012 Elsevier B.V. All rights reserved.

Item Type: Paper
Uncontrolled Keywords: mesenchymal stem-cells smooth-muscle ng2 proteoglycan progenitor cells microvascular pericytes satellite cells blood-vessels bone-marrow dental-pulp neural stem
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > green fluorescent protein
therapies > stem cells
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > transgenic animal
CSHL Authors:
Communities: CSHL labs > Enikopolov lab
CSHL Cancer Center Shared Resources > Animal Services
Depositing User: Matt Covey
Date: January 2013
Date Deposited: 29 Mar 2013 19:00
Last Modified: 26 Dec 2014 15:00
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28068

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