Pick one, but be quick: 5′ splice sites and the problems of too many choices

Roca, X., Krainer, A. R., Eperon, I. C. (January 2013) Pick one, but be quick: 5′ splice sites and the problems of too many choices. Genes and Development, 27 (2). pp. 129-144. ISSN 08909369 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/23348838
DOI: 10.1101/gad.209759.112

Abstract

Splice site selection is fundamental to pre-mRNA splicing and the expansion of genomic coding potential. 5′ Splice sites (5′ss) are the critical elements at the 5′ end of introns and are extremely diverse, as thousands of different sequences act as bona fide 5′ss in the human transcriptome. Most 5′ss are recognized by base-pairing with the 5′ end of the U1 small nuclear RNA (snRNA). Here we review the history of research on 5′ss selection, highlighting the difficulties of establishing how basepairing strength determines splicing outcomes. We also discuss recent work demonstrating that U1 snRNA:5′ss helices can accommodate noncanonical registers such as bulged duplexes. In addition, we describe the mechanisms by which other snRNAs, regulatory proteins, splicing enhancers, and the relative positions of alternative 5′ss contribute to selection. Moreover, we discuss mechanisms by which the recognition of numerous candidate 5′ss might lead to selection of a single 5′ss and propose that protein complexes propagate along the exon, thereby changing its physical behavior so as to affect 5′ss selection. © 2013 by Cold Spring Harbor Laboratory Press.

Item Type: Paper
Uncontrolled Keywords: 5′ splice sites Exons Pre-mRNA Splicing U1 snRNA
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > pre-mRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > snRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > splice site
CSHL Authors:
Communities: CSHL labs > Krainer lab
Depositing User: Matt Covey
Date: January 2013
Date Deposited: 29 Mar 2013 16:40
Last Modified: 29 Mar 2013 16:40
PMCID: PMC3566305
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28061

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