Distinct mechanisms determine transposon inheritance and methylation via small interfering RNA and histone modification

Lippman, Z., May, B., Yordan, C., Singer, T., Martienssen, R. (2003) Distinct mechanisms determine transposon inheritance and methylation via small interfering RNA and histone modification. PLoS biology, 1 (3). pp. 420-428. ISSN 1545-7885 (Electronic)1544-9173 (Linking)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/14691539
DOI: 10.1371/journal.pbio.0000067

Abstract

Heritable, but reversible, changes in transposable element activity were first observed in maize by Barbara McClintock in the 1950s. More recently, transposon silencing has been associated with DNA methylation, histone H3 lysine-9 methylation (H3mK9), and RNA interference (RNAi). Using a genetic approach, we have investigated the role of these modifications in the epigenetic regulation and inheritance of six Arabidopsis transposons. Silencing of most of the transposons is relieved in DNA methyltransferase (met1), chromatin remodeling ATPase (ddm1), and histone modification (sil1) mutants. In contrast, only a small subset of the transposons require the H3mK9 methyltransferase KRYPTONITE, the RNAi gene ARGONAUTE1, and the CXG methyltransferase CHROMOMETHYLASE3. In crosses to wild-type plants, epigenetic inheritance of active transposons varied from mutant to mutant, indicating these genes differ in their ability to silence transposons. According to their pattern of transposon regulation, the mutants can be divided into two groups, which suggests that there are distinct, but interacting, complexes or pathways involved in transposon silencing. Furthermore, different transposons tend to be susceptible to different forms of epigenetic regulation.

Item Type: Paper
Uncontrolled Keywords: Adenosine Triphosphatases genetics Arabidopsis genetics Arabidopsis Proteins genetics metabolism Argonaute Proteins Chromatin metabolism Chromatin Immunoprecipitation Computational Biology DNA (Cytosine-5-)-Methyltransferase genetics DNA Methylation DNA Primers chemistry DNA Transposable Elements DNA-Binding Proteins metabolism DNA-Cytosine Methylases Gene Expression Regulation Plant Gene Silencing Genes Plant Histone-Lysine N-Methyltransferase genetics Histones metabolism Models Genetic Mutation Open Reading Frames Polymerase Chain Reaction RNA Interference RNA Small Interfering genetics Transcription Factors metabolism Transcription Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein methylation > histone methylation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein methylation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > siRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transposons
CSHL Authors:
Communities: CSHL labs > Lippman lab
CSHL labs > Martienssen lab
Watson School > Publications
Depositing User: Matt Covey
Date Deposited: 27 Mar 2013 16:47
Last Modified: 19 Sep 2014 14:43
PMCID: PMC300680
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28024

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