Maize genome sequencing by methylation filtrations

Palmer, L. E., Rabinowicz, P. D., O'Shaughnessy, A. L., Balija, V. S., Nascimento, L. U., Dike, S., de la Bastide, M., Martienssen, R. A., McCombie, W. R. (2003) Maize genome sequencing by methylation filtrations. Science, 302 (5653). pp. 2115-2117. ISSN 0036-8075

URL: http://www.ncbi.nlm.nih.gov/pubmed/14684820
DOI: 10.1126/science.1091265

Abstract

Gene enrichment strategies offer an alternative to sequencing large and repetitive genomes such as that of maize. We report the generation and analysis of nearly 100,000 undermethylated (or methylation filtration) maize sequences. Comparison with the rice genome reveals that methylation filtration results in a more comprehensive representation of maize genes than those that result from expressed sequence tags or transposon insertion sites sequences. About 7% of the repetitive DNA is unmethylated and thus selected in our libraries, but potentially active transposons and unmethylated organelle genomes can be identified. Reverse transcription polymerase chain reaction can be used to finish the maize transcriptome.

Item Type: Paper
Uncontrolled Keywords: DNA methylation transposable elements draft sequence gene retrotransposons grasses regions plants
Subjects: bioinformatics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
Investigative techniques and equipment
organism description > plant > maize
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > genomes
organism description > plant
CSHL Authors:
Communities: CSHL labs > Martienssen lab
CSHL labs > McCombie lab
Depositing User: Matt Covey
Date Deposited: 27 Mar 2013 16:05
Last Modified: 27 Mar 2013 16:05
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28021

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