Genes and transposons are differentially methylated in plants, but not in mammals

Rabinowicz, P. D., Palmer, L. E., May, B. P., Hemann, M. T., Lowe, S. W., McCombie, W. R., Martienssen, R. A. (2003) Genes and transposons are differentially methylated in plants, but not in mammals. Genome Research, 13 (12). pp. 2658-64. ISSN 1088-9051

URL: http://www.ncbi.nlm.nih.gov/pubmed/14656970
DOI: 10.1101/gr.1784803

Abstract

DNA methylation is found in many eukaryotes, but its function is still controversial. We have studied the methylation of plant and animal genomes using a PCR-based technique amenable for high throughput. Repetitive elements are methylated in both organisms, but whereas most mammalian exons are methylated, plant exons are not. Thus, targeting of methylation specifically to transposons appears to be restricted to plants. We propose that the mechanistic basis of this difference may involve RNA interference. Sequencing strategies that depend on differential methylation are predicted to have different outcomes in plant and mammalian genomes.

Item Type: Paper
Uncontrolled Keywords: Animals DNA Methylation DNA Transposable Elements Fibroblasts chemistry metabolism Genes Genes Plant Genome Genome Human Genome Plant Humans Lung cytology embryology Mice Mice Inbred C57BL Mice Transgenic Spleen chemistry embryology metabolism Zea mays/genetics
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA methylation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
organism description > animal
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
organism description > plant
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transposons
CSHL Authors:
Communities: CSHL labs > Lowe lab
CSHL labs > Martienssen lab
CSHL labs > McCombie lab
Depositing User: Matt Covey
Date Deposited: 01 Apr 2013 18:56
Last Modified: 01 Apr 2013 18:56
PMCID: PMC403807
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28012

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