Role of the inhibitory DNA-binding surface of human TATA-binding protein in recruitment of human TFIIB family members

Zhao, X., Herr, W. (November 2003) Role of the inhibitory DNA-binding surface of human TATA-binding protein in recruitment of human TFIIB family members. Molecular and Cellular Biology, 23 (22). pp. 8152-60. ISSN 0270-7306 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/14585974
DOI: 10.1128/​MCB.23.22.8152-8160.2003

Abstract

TATA box recognition by TATA-binding protein (TBP) is a key step in transcriptional initiation complex assembly on TATA-box-containing RNA polymerase (Pol) II and III promoters. This process is inhibited by the inhibitory DNA-binding (IDB) surface on the human TBP core domain (TBP(CORE)) and is stimulated by promoter-specific basal transcription factors, such as two human TFIIB family members, the Pol II factor TFIIB and the Pol III factor Brf2, which is required for transcription from TATA-box-containing Pol III promoters. In contrast, the third TFIIB family member, Brf1, which is required for transcription from TATA-less Pol III promoters, does not stimulate TBP binding to the TATA box. We show here that in addition to its role in regulating TBP binding to a TATA box, the TBP IDB surface is unexpectedly involved in TBP association with all three TFIIB family members. Interestingly, the loss of IDB function has specific and diverse effects on each TFIIB family member. Indeed, the IDB and prototypical TFIIB contact surfaces of TBP, which lie on opposite sides of the TBP(CORE), cooperate to form the wild-type TFIIB-TBP-TATA box complex. These results reveal how, through differential usage of opposite surfaces of the TBP(CORE), TBP can achieve versatility in the assembly of Pol II and Pol III promoter complexes with TFIIB family proteins.

Item Type: Paper
Uncontrolled Keywords: Binding Sites DNA/genetics/metabolism Humans Macromolecular Substances Models, Molecular Mutagenesis, Site-Directed Promoter Regions (Genetics) RNA, Small Nuclear/genetics/metabolism Recombinant Fusion Proteins/chemistry/genetics/metabolism TATA Box TATA-Box Binding Protein/ chemistry/genetics/ metabolism Transcription Factor TFIIB/ chemistry/genetics/ metabolism Transcription Factors/genetics/metabolism Transcription, Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
CSHL Authors:
Communities: CSHL labs > Herr lab
Depositing User: Matt Covey
Date: November 2003
Date Deposited: 28 Mar 2013 13:20
Last Modified: 28 Mar 2013 13:20
PMCID: PMC262358
Related URLs:
URI: http://repository.cshl.edu/id/eprint/28000

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving