Protein components of mitochondrial DNA nucleoids in higher eukaryotes

Bogenhagen, D. F., Wang, Y. S., Shen, E. L., Kobayashi, R. (November 2003) Protein components of mitochondrial DNA nucleoids in higher eukaryotes. Molecular & Cellular Proteomics, 2 (11). pp. 1205-1216. ISSN 1535-9476

URL: http://www.ncbi.nlm.nih.gov/pubmed/14514796
DOI: 10.1074/mcp.M300035-MCP200

Abstract

Mitochondrial DNA ( mtDNA) is not packaged in nucleosomal particles, but has been reported to associate with the mitochondrial inner membrane. Gentle lysis of Xenopus oocyte mitochondria with nonionic detergent liberates a nucleoprotein complex containing mtDNA associated with a previously characterized DNA binding partner, mitochondrial transcription factor A ( mtTFA), as well as a series of inner membrane proteins identified by sequencing. More extensive detergent treatment stripped most of these proteins from the DNA, leaving a limited number of proteins in a nucleoid core. Sequencing of the major proteins retained in association with mtDNA revealed the expected mtDNA binding proteins, mtTFA and mitochondrial single-stranded DNA binding protein ( mtSSB), as well as four proteins not previously reported to associate with mtDNA. These include adenine nucleotide translocator 1, the lipoyl-containing E2 subunits of pyruvate dehydrogenase and branched chain alpha-ketoacid dehydrogenase and prohibitin 2. The association of several of these proteins with mtTFA-containing mtDNA nucleoids was confirmed by immunoprecipitation.

Item Type: Paper
Uncontrolled Keywords: PRIMARY BILIARY-CIRRHOSIS XENOPUS-LAEVIS OOCYTES BINDING-PROTEIN SACCHAROMYCES-CEREVISIAE PYRUVATE-DEHYDROGENASE POLYACRYLAMIDE GELS YEAST MITOCHONDRIA PROHIBITIN FAMILY SILVER STAIN E2 SUBUNIT
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
organs, tissues, organelles, cell types and functions > organelles, types and functions > mitochondria
organs, tissues, organelles, cell types and functions > organelles, types and functions
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
organism description > animal > Frog > xenopus
CSHL Authors:
Communities: CSHL labs > Kobayashi lab
Depositing User: Matt Covey
Date: November 2003
Date Deposited: 01 Apr 2013 20:01
Last Modified: 01 Apr 2013 20:01
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27986

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving