Nitric oxide negatively regulates mammalian adult neurogenesis

Packer, M. A., Stasiv, Y., Benraiss, A., Chmielnicki, E., Grinberg, A., Westphal, H., Goldman, S. A., Enikolopov, G. (2003) Nitric oxide negatively regulates mammalian adult neurogenesis. Proceedings of the National Academy of Sciences of the United States of America, 100 (16). pp. 9566-9571. ISSN 0027-8424

URL: http://www.ncbi.nlm.nih.gov/pubmed/12886012
DOI: 10.1073/pnas.1633579100

Abstract

Neural progenitor cells are widespread throughout the adult central nervous system but only give rise to neurons in specific loci. Negative regulators of neurogenesis have therefore been postulated, but none have yet been identified as subserving a significant role in the adult brain. Here we report that nitric oxide (NO) acts as an important negative regulator of cell proliferation in the adult mammalian brain. We used two independent approaches to examine the function of NO in adult neurogenesis. In a pharmacological approach, we suppressed NO production in the rat brain by intraventricular infusion of an NO synthase inhibitor. In a genetic approach, we generated a null mutant neuronal NO synthase knockout mouse line by targeting the exon encoding active center of the enzyme. In both models, the number of new cells generated in neurogenic areas of the adult brain, the olfactory subependyma and the dentate gyrus, was strongly augmented, which indicates that division of neural stem cells in the adult brain is controlled by NO and suggests a strategy for enhancing neurogenesis in the adult central nervous system.

Item Type: Paper
Uncontrolled Keywords: NEURAL STEM-CELLS FOCAL CEREBRAL-ISCHEMIA RAT BRAIN NEUROTROPHIC FACTOR GROWTH-FACTOR NEURONAL DIFFERENTIATION DROSOPHILA DEVELOPMENT SUBVENTRICULAR ZONE PRECURSOR CELLS S-NITROSYLATION
Subjects: therapies > stem cells > Adult
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > germ cell > Adult
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > germ cell > Adult
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > germ cell > Adult
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > Adult
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > Adult
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > Adult
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > Adult
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > Adult
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > Adult

organism description > animal > developmental stage > adult
organism description > animal
organs, tissues, organelles, cell types and functions > cell types and functions > cell types
organs, tissues, organelles, cell types and functions > cell types and functions > cell types
organs, tissues, organelles, cell types and functions > cell types and functions > cell types

organs, tissues, organelles, cell types and functions > cell types and functions
organism description > animal > developmental stage
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons

bioinformatics > genomics and proteomics > small molecules > nitric oxide
organism description > animal > mammal > rodent > rat
organism description > animal > mammal > rodent > rat

bioinformatics > genomics and proteomics > small molecules
therapies > stem cells
CSHL Authors:
Communities: CSHL labs > Enikopolov lab
Depositing User: Matt Covey
Date Deposited: 02 Apr 2013 15:55
Last Modified: 02 Apr 2013 15:55
PMCID: PMC170958
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27965

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving