The ATP-waiting conformation of rotating F1-ATPase revealed by single-pair fluorescence resonance energy transfer

Yasuda, R., Masaike, T., Adachi, K., Noji, H., Itoh, H., Kinosita, K. (August 2003) The ATP-waiting conformation of rotating F1-ATPase revealed by single-pair fluorescence resonance energy transfer. Proceedings of the National Academy of Sciences of the United States of America, 100 (16). pp. 9314-8. ISSN 0027-8424

URL: http://www.ncbi.nlm.nih.gov/pubmed/12876203
DOI: 10.1073/pnas.1637860100

Abstract

F1-ATPase is an ATP-driven rotary motor in which a rod-shaped gamma subunit rotates inside a cylinder made of alpha3beta3 subunits. To elucidate the conformations of rotating F1, we measured fluorescence resonance energy transfer (FRET) between a donor on one of the three betas and an acceptor on gamma in single F1 molecules. The yield of FRET changed stepwise at low ATP concentrations, reflecting the stepwise rotation of gamma. In the ATP-waiting state, the FRET yields indicated a gamma position approximately 40 degrees counterclockwise (= direction of rotation) from that in the crystal structures of mitochondrial F1, suggesting that the crystal structures mimic a metastable state before product release.

Item Type: Paper
Uncontrolled Keywords: Adenosine Triphosphatases/chemistry Adenosine Triphosphate/ chemistry Bacillus/enzymology Crystallography, X-Ray Dose-Response Relationship, Drug Fluorescence Resonance Energy Transfer Models, Biological Models, Theoretical Mutation Protein Conformation Proton-Translocating ATPases/ chemistry
Subjects: bioinformatics > genomics and proteomics > small molecules > ATP
bioinformatics > genomics and proteomics > small molecules
CSHL Authors:
Communities: CSHL labs > Svoboda lab
Depositing User: Matt Covey
Date: 5 August 2003
Date Deposited: 11 Jun 2013 19:13
Last Modified: 11 Jun 2013 19:13
PMCID: PMC170915
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27961

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving