A global transcriptional regulatory role for c-Myc in Burkitt's lymphoma cells

Li, Z. R., Van Calcar, S., Qu, C. X., Cavenee, W. K., Zhang, M. Q., Ren, B. (July 2003) A global transcriptional regulatory role for c-Myc in Burkitt's lymphoma cells. Proceedings of the National Academy of Sciences of the United States of America, 100 (14). pp. 8164-8169. ISSN 0027-8424

URL: http://www.ncbi.nlm.nih.gov/pubmed/12808131
DOI: 10.1073/pnas.1332764100

Abstract

Overexpression of c-Myc is one of the most common alterations in human cancers, yet it is not clear how this transcription factor acts to promote malignant transformation. To understand the molecular targets of c-Myc function, we have used an unbiased genome-wide location-analysis approach to examine the genomic binding sites of c-Myc in Burkitt's lymphoma cells. We find that c-Myc together with its heterodimeric partner, Max, occupy >15% of gene promoters tested in these cancer cells. The DNA binding of c-Myc and Max correlates extensively with gene expression throughout the genome, a hallmark attribute of general transcription factors. The c-Myc/Max heterodimer complexes also colocalize with transcription factor IID in these cells, further supporting a general role for overexpressed c-Myc in global gene regulation. In addition, transcription of a majority of c-Myc target genes exhibits changes correlated with levels of c-myc mRNA in a diverse set of tissues and cell lines, supporting the conclusion that c-Myc regulates them. Taken together, these results suggest a general role for overexpressed c-Myc in global transcriptional regulation in some cancer cells and point toward molecular mechanisms for c-Myc function in malignant transformation.

Item Type: Paper
Uncontrolled Keywords: TATA-BINDING PROTEIN SACCHAROMYCES-CEREVISIAE TRANSGENIC MICE DNA-BINDING GENE ASSOCIATION ONCOGENE YEAST TRANSFORMATION TUMORIGENESIS
Subjects: diseases & disorders > cancer
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > Myc
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types
diseases & disorders > cancer > cancer types > lymphoma
diseases & disorders > cancer > cancer types
CSHL Authors:
Communities: CSHL labs > Zhang lab
Depositing User: Matt Covey
Date: July 2003
Date Deposited: 26 Jun 2013 19:11
Last Modified: 26 Jun 2013 19:11
PMCID: PMC166200
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27945

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving