Muskett, P. R., Clissold, L., Marocco, A., Springer, P. S., Martienssen, R., Dean, C. (June 2003) A resource of mapped dissociation launch pads for targeted insertional mutagenesis in the Arabidopsis genome. Plant physiology, 132 (2). pp. 506-16. ISSN 0032-0889 (Print)0032-0889 (Linking)
Abstract
We describe a new resource for targeted insertional mutagenesis in Arabidopsis using a maize (Zea mays) Activator/Dissociation (Ds) two-element system. The two components of the system, T-DNA vectors carrying a Ds launch pad and a stable Activator transposase source, were designed to simplify selection of transposition events and maximize their usefulness. Because Ds elements preferentially transpose to nearby genomic sites, they can be used in targeted mutagenesis of linked genes. To efficiently target all genes throughout the genome, we generated a large population of transgenic Arabidopsis plants containing the Ds launch pad construct, identified lines containing single Ds launch pad inserts, and mapped the positions of Ds launch pads in 89 lines. The integration sites of the Ds launch pads were relatively evenly distributed on all five chromosomes, except for a region of chromosomes 2 and 4 and the centromeric regions. This resource therefore provides access to the majority of the Arabidopsis genome for targeted tagging.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Agrobacterium tumefaciens genetics Arabidopsis drug effects genetics Base Sequence DNA Primers DNA Bacterial genetics DNA Plant genetics Genome Plant Herbicides pharmacology Mutagenesis Insertional Tobacco genetics Transformation Genetic |
| Subjects: | organism description > plant > Arabidopsis bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics > genetics & nucleic acid processing > genomes bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations > mutagenesis organism description > plant |
| CSHL Authors: | |
| Communities: | CSHL labs > Martienssen lab |
| Depositing User: | Matt Covey |
| Date: | June 2003 |
| Date Deposited: | 11 Jun 2013 20:52 |
| Last Modified: | 11 Jun 2013 20:52 |
| PMCID: | PMC166993 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/27943 |
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