A resource of mapped dissociation launch pads for targeted insertional mutagenesis in the Arabidopsis genome

Muskett, P. R., Clissold, L., Marocco, A., Springer, P. S., Martienssen, R., Dean, C. (2003) A resource of mapped dissociation launch pads for targeted insertional mutagenesis in the Arabidopsis genome. Plant physiology, 132 (2). pp. 506-16. ISSN 0032-0889 (Print)0032-0889 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/12805583
DOI: 10.1104/pp.102.016535

Abstract

We describe a new resource for targeted insertional mutagenesis in Arabidopsis using a maize (Zea mays) Activator/Dissociation (Ds) two-element system. The two components of the system, T-DNA vectors carrying a Ds launch pad and a stable Activator transposase source, were designed to simplify selection of transposition events and maximize their usefulness. Because Ds elements preferentially transpose to nearby genomic sites, they can be used in targeted mutagenesis of linked genes. To efficiently target all genes throughout the genome, we generated a large population of transgenic Arabidopsis plants containing the Ds launch pad construct, identified lines containing single Ds launch pad inserts, and mapped the positions of Ds launch pads in 89 lines. The integration sites of the Ds launch pads were relatively evenly distributed on all five chromosomes, except for a region of chromosomes 2 and 4 and the centromeric regions. This resource therefore provides access to the majority of the Arabidopsis genome for targeted tagging.

Item Type: Paper
Uncontrolled Keywords: Agrobacterium tumefaciens genetics Arabidopsis drug effects genetics Base Sequence DNA Primers DNA Bacterial genetics DNA Plant genetics Genome Plant Herbicides pharmacology Mutagenesis Insertional Tobacco genetics Transformation Genetic
Subjects: organism description > plant > Arabidopsis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > genomes
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations > mutagenesis
organism description > plant
CSHL Authors:
Communities: CSHL labs > Martienssen lab
Depositing User: Matt Covey
Date Deposited: 11 Jun 2013 20:52
Last Modified: 11 Jun 2013 20:52
PMCID: PMC166993
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27943

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