Phosphorylation-regulated cleavage of the tumor suppressor PTEN by caspase-3 - Implications for the control of protein stability and PTEN-protein interactions

Torres, J., Rodriguez, J., Myers, M. P., Valiente, M., Graves, J. D., Tonks, N. K., Pulido, R. (August 2003) Phosphorylation-regulated cleavage of the tumor suppressor PTEN by caspase-3 - Implications for the control of protein stability and PTEN-protein interactions. Journal of Biological Chemistry, 278 (33). pp. 30652-30660. ISSN 0021-9258

URL: http://www.ncbi.nlm.nih.gov/pubmed/12788938
DOI: 10.1074/jbc.M212610200

Abstract

PTEN phosphatase is one of the most commonly targeted tumor suppressors in human cancers and a key regulator of cell growth and apoptosis. We have found that PTEN is cleaved by caspase-3 at several target sites, located in unstructured regions within the C terminus of the molecule. Cleavage of PTEN was increased upon TNFalpha-cell treatment and was negatively regulated by phosphorylation of the C-terminal tail of PTEN by the protein kinase CK2. The proteolytic PTEN fragments displayed reduced protein stability, and their capability to interact with the PTEN interacting scaffolding protein S-SCAM/MAGI-2 was lost. Interestingly, S-SCAM/MAGI-2 was also cleaved by caspase-3. Our findings suggest the existence of a regulatory mechanism of protein stability and PTEN-protein interactions during apoptosis, executed by caspase-3 in a PTEN phosphorylation-regulated manner.

Item Type: Paper
Uncontrolled Keywords: PDZ DOMAIN CELL-SURVIVAL KINASE CK2 PHOSPHATASE APOPTOSIS GENE PTEN/MMAC1 MAGI-2 DEATH SITES
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > PTEN
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > protein phosphatase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
CSHL Authors:
Communities: CSHL labs > Tonks lab
Depositing User: Matt Covey
Date: August 2003
Date Deposited: 11 Jun 2013 20:02
Last Modified: 11 Jun 2013 20:02
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27934

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