Proteolytic processing is necessary to separate and ensure proper cell growth and cytokinesis functions of HCF-1

Julien, E., Herr, W. (2003) Proteolytic processing is necessary to separate and ensure proper cell growth and cytokinesis functions of HCF-1. Embo Journal, 22 (10). pp. 2360-2369. ISSN 0261-4189

URL: http://www.ncbi.nlm.nih.gov/pubmed/12743030
DOI: 10.1093/emboj/cdg242

Abstract

HCF-1 is a highly conserved and abundant chromatin-associated host cell factor required for transcriptional activation of herpes simplex virus immediate-early genes by the virion protein VP16. HCF-1 exists as a heterodimeric complex of associated N- (HCF-1(N)) and C- (HCF-1(C)) terminal subunits that result from proteolytic processing of a precursor protein. We have used small-interfering RNA (siRNA) to inactivate HCF-1 in an array of normal and transformed mammalian cells to identify its cellular functions. Our results show that HCF-1 is a broadly acting regulator of two stages of the cell cycle: exit from mitosis, where it ensures proper cytokinesis, and passage through the G(1) phase, where it promotes cell cycle progression. Proteolytic processing is necessary to separate and ensure these two HCF-1 activities, which are performed by separate HCF-1 subunits: the HCF-1(N) subunit promotes passage through the G(1) phase whereas the HCF-1(C) subunit is involved in proper exit from mitosis. These results suggest that HCF-1 links the regulation of exit from mitosis and the G(1) phase of cell growth, possibly to coordinate the reactivation of gene expression after mitosis.

Item Type: Paper
Uncontrolled Keywords: cell cycle herpes simplex virus mitosis proteolysis siRNA silencing TRANSCRIPTIONAL REGULATION ACTIVATION DOMAIN CARCINOMA-CELLS VP16 PROTEIN COMPLEX FAMILY PHOSPHORYLATION TRANSACTIVATION PROLIFERATION
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
organism description > virus > herpes simplex virus
organs, tissues, organelles, cell types and functions > organelles, types and functions > mitosis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > siRNA
organism description > virus
CSHL Authors:
Communities: CSHL labs > Herr lab
Depositing User: Matt Covey
Date Deposited: 27 Jun 2013 14:23
Last Modified: 27 Jun 2013 14:23
PMCID: PMC156000
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27926

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