Crystal structure of the Drosophila Mago nashi-Y14 complex

Shi, H., Xu, R. M. (April 2003) Crystal structure of the Drosophila Mago nashi-Y14 complex. Genes & Development, 17 (8). pp. 971-976. ISSN 0890-9369

URL: http://www.ncbi.nlm.nih.gov/pubmed/12704080
DOI: 10.1101/gad.260403

Abstract

Pre-mRNA splicing is essential for generating mature mRNA and is also important for subsequent mRNA export and quality control. The splicing history is imprinted on spliced mRNA through the deposition of a splicing-dependent multiprotein complex, the exon junction complex (EJC), at similar to20 nucleotides upstream of exon-exon junctions. The EJC is a dynamic structure containing proteins functioning in the nuclear export and nonsense-mediated decay of spliced mRNAs. Mago nashi (Mago) and Y14 are core components of the EJC, and they form a stable heterodimer that strongly associates with spliced mRNA. Here we report a 1.85 Angstrom-resolution structure of the Drosophila Mago-Y14 complex. Surprisingly, the structure shows that the canonical RNA-binding surface of the Y14 RNA recognition motif (RRM) is involved in extensive protein-protein interactions with Mago. This unexpected finding provides important insights for understanding the molecular mechanisms of EJC assembly and RRM-mediated protein-protein interactions.

Item Type: Paper
Uncontrolled Keywords: Mago Y14 RNA recognition motif splicing nuclear export nonsense-mediated decay EXON JUNCTION COMPLEX MESSENGER-RNA SURVEILLANCE BINDING-PROTEIN RECOGNITION EXPORT CYTOPLASM OOCYTE Y14 ASSOCIATION POSTERIOR
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
organism description > animal > insect > Drosophila
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > pre-mRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA splicing
CSHL Authors:
Communities: CSHL labs > Xu lab
Depositing User: Matt Covey
Date: April 2003
Date Deposited: 01 Jul 2013 15:45
Last Modified: 01 Jul 2013 15:45
PMCID: PMC196043
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27916

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