Experience strengthening transmission by driving AMPA receptors into synapses

Takahashi, T., Svoboda, K., Malinow, R. (March 2003) Experience strengthening transmission by driving AMPA receptors into synapses. Science, 299 (5612). pp. 1585-1588. ISSN 0036-8075

URL: http://www.ncbi.nlm.nih.gov/pubmed/12624270
DOI: 10.1126/science.1079886

Abstract

The mechanisms underlying experience-dependent plasticity in the brain may depend on the AMPA subclass of glutamate receptors (AMPA-Rs). We examined the trafficking of AMPA-Rs into synapses in the developing rat barrel cortex. In vivo gene delivery was combined with in vitro recordings to show that experience drives recombinant GluR1, an AMPA-R subunit, into synapses formed between layer 4 and layer 2/3 neurons. Moreover, expression of the GluR1 cytoplasmic tail, a construct that inhibits synaptic delivery of endogenous AMPA-Rs during long-term potentiation, blocked experience-driven synaptic potentiation. In general, synaptic incorporation of AMPA-Rs in vivo conforms to rules identified in vitro and contributes to plasticity driven by natural stimuli in the mammalian brain.

Item Type: Paper
Uncontrolled Keywords: BARREL FIELD CORTEX SYNAPTIC PLASTICITY EXCITATORY SYNAPSES HIPPOCAMPAL-NEURONS IN-VIVO TRAFFICKING RAT POTENTIATION EXPRESSION MECHANISM
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > AMPA receptor
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > synaptic transmission
CSHL Authors:
Communities: CSHL labs > Malinow lab
CSHL labs > Svoboda lab
Depositing User: Matt Covey
Date: March 2003
Date Deposited: 01 Jul 2013 18:43
Last Modified: 01 Jul 2013 18:43
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27898

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