A shared surface of TBP directs RNA polymerase II and III transcription via association with different TFIIB family members

Zhao, X. M., Schramm, L., Hernandez, N., Herr, W. (January 2003) A shared surface of TBP directs RNA polymerase II and III transcription via association with different TFIIB family members. Molecular Cell, 11 (1). pp. 151-161. ISSN 1097-2765

URL: http://www.ncbi.nlm.nih.gov/pubmed/12535529
DOI: 10.1016/S1097-2765(02)00797-9

Abstract

The TATA box binding protein TBP is highly conserved and the only known basal factor that is involved in transcription by all three eukaryotic nuclear RNA polymerases from promoters with or without a TATA box. By mutagenesis and analysis on a selected set of four model pol II and pol III TATA box-containing and TATA-less promoters, we demonstrate that human TBP utilizes two modes to achieve its versatile functions. First, it uses a different set of surfaces on the conserved and structured TBP core domain to direct transcription from each of the four model promoters. Second, unlike yeast TBP, human TBP can use a shared surface to interact with two different TFIIB family members-TFIIB and Brf2-to initiate transcription by different RNA polymerases.

Item Type: Paper
Uncontrolled Keywords: TATA-BOX-BINDING YEAST TFIIA/TBP/DNA COMPLEX CRYSTAL-STRUCTURE IN-VIVO PREINITIATION COMPLEX PROTEIN PROMOTERS ELEMENT INITIATION GENES
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > RNA polymerase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes
CSHL Authors:
Communities: CSHL labs > Herr lab
Depositing User: Matt Covey
Date: January 2003
Date Deposited: 01 Jul 2013 19:22
Last Modified: 01 Jul 2013 19:22
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27873

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