RNA interference machinery regulates chromosome dynamics during mitosis and meiosis in fission yeast

Hall, I. M., Noma, K., Grewal, S. I. (2003) RNA interference machinery regulates chromosome dynamics during mitosis and meiosis in fission yeast. Proceedings of the National Academy of Sciences of the United States of America, 100 (1). pp. 193-8. ISSN 0027-8424 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/12509501
DOI: 10.1073/pnas.232688099

Abstract

The regulation of higher-order chromosome structure is central to cell division and sexual reproduction. Heterochromatin assembly at the centromeres facilitates both kinetochore formation and sister chromatid cohesion, and the formation of specialized chromatin structures at telomeres serves to maintain the length of telomeric repeats, to suppress recombination, and to aid in formation of a bouquet-like structure that facilitates homologous chromosome pairing during meiosis. In fission yeast, genes encoding the Argonaute, Dicer, and RNA-dependent RNA polymerase factors involved in RNA interference (RNAi) are required for heterochromatin formation at the centromeres and mating type region. In this study, we examine the effects of deletions of the fission yeast RNAi machinery on chromosome dynamics during mitosis and meiosis. We find that the RNAi machinery is required for the accurate segregation of chromosomes. Defects in mitotic chromosome segregation are correlated with loss of cohesin at centromeres. Although the telomeres of RNAi mutants maintain silencing, length, and localization of the heterochromatin protein Swi6, we discovered defects in the proper clustering of telomeres in interphase mitotic cells. Furthermore, a small proportion of RNAi mutant cells display aberrant telomere clustering during meiotic prophase. This study demonstrates that the fission yeast RNAi machinery is required for the proper regulation of chromosome architecture during mitosis and meiosis.

Item Type: Paper
Uncontrolled Keywords: Chromosomes, Fungal Gene Expression Regulation, Fungal Gene Silencing Meiosis Mitosis RNA, Small Interfering/ genetics Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Schizosaccharomyces/ cytology/ genetics Telomere/genetics
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function
organs, tissues, organelles, cell types and functions > organelles, types and functions > meiosis
organs, tissues, organelles, cell types and functions > organelles, types and functions > mitosis
organs, tissues, organelles, cell types and functions > organelles, types and functions
organism description > yeast
CSHL Authors:
Communities: CSHL labs > Grewal lab
Watson School > Publications
Depositing User: Matt Covey
Date Deposited: 02 Jul 2013 13:38
Last Modified: 19 Sep 2014 14:38
PMCID: PMC140924
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27868

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