Draft genome of the filarial nematode parasite Brugia malayi

Ghedin, E., Wang, S., Spiro, D., Caler, E., Zhao, Q., Crabtree, J., Allen, J. E., Delcher, A. L., Guiliano, D. B., Miranda-Saavedra, D., Angiuoli, S. V., Creasy, T., Amedeo, P., Haas, B., El-Sayed, N. M., Wortman, J. R., Feldblyum, T., Tallon, L., Schatz, M., Shumway, M., Koo, H., Salzberg, S. L., Schobel, S., Pertea, M., Pop, M., White, O., Barton, G. J., Carlow, C. K. S., Crawford, M. J., Daub, J., Dimmic, M. W., Estes, C. F., Foster, J. M., Ganatra, M., Gregory, W. F., Johnson, N. M., Jin, M., Komuniecki, R., Korf, I., Kumar, S., Laney, S., Li, B. W., Li, W., Lindblom, T. H., Lustigman, S., Ma, D., Maina, C. V., Martin, D. M. A., McCarter, J. P., McReynolds, L., Mitreva, M., Nutman, T. B., Parkinson, J., Peregrín-Alvarez, J. M., Poole, C., Ren, Q., Saunders, L., Sluder, A. E., Smith, K., Stanke, M., Unnasch, T. R., Ware, J., Wei, A. D., Weil, G., Williams, D. J., Zhang, Y., Williams, S. A., Fraser-Liggett, C., Slatko, B., Blaxter, M. L., Scott, A. L. (2007) Draft genome of the filarial nematode parasite Brugia malayi. Science, 317 (5845). pp. 1756-1760. ISSN 00368075 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/17885136
DOI: 10.1126/science.1145406

Abstract

Parasitic nematodes that cause elephantiasis and river blindness threaten hundreds of millions of people in the developing world. We have sequenced the ∼90 megabase (Mb) genome of the human filarial parasite Brugia malayi and predict ∼11,500 protein coding genes in 71 Mb of robustly assembled sequence. Comparative analysis with the free-living, model nematode Caenorhabditis elegans revealed that, despite these genes having maintained little conservation of local synteny during ∼350 million years of evolution, they largely remain in linkage on chromosomal units. More than 100 conserved operons were identified. Analysis of the predicted proteome provides evidence for adaptations of B. malayi to niches in its human and vector hosts and insights into the molecular basis of a mutualistic relationship with its Wolbachia endosymbiont. These findings offer a foundation for rational drug design.

Item Type: Paper
Uncontrolled Keywords: comparative study developing world genome nematode parasite proteomics article Brugia malayi Caenorhabditis elegans chromosome analysis controlled study drug design elephantiasis endosymbiont Filaria gene identification gene sequence genetic analysis genetic linkage genome analysis molecular evolution nonhuman nucleotide sequence operon prediction priority journal species comparison synteny Animals Caenorhabditis Drosophila melanogaster Drug Resistance Filariasis Genome, Helminth Humans Molecular Sequence Data Nematoda Wolbachia
Subjects: bioinformatics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > genomes
CSHL Authors:
Communities: CSHL labs > Schatz lab
Depositing User: Matt Covey
Date Deposited: 15 Mar 2013 18:11
Last Modified: 15 Mar 2013 18:11
PMCID: PMC2613796
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27824

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