Profile of histone lysine methylation across transcribed mammalian chromatin

Vakoc, C. R., Sachdeva, M. M., Wang, H., Blobel, G. A. (2006) Profile of histone lysine methylation across transcribed mammalian chromatin. Molecular and Cellular Biology, 26 (24). pp. 9185-9195. ISSN 02707306 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/17030614
DOI: 10.1128/mcb.01529-06

Abstract

Complex patterns of histone lysine methylation encode distinct functions within chromatin. We previously reported that trimethylation of lysine 9 of histone H3 (H3K9) occurs at both silent heterochromatin and at the transcribed regions of active mammalian genes, suggesting that the extent of histone lysine methylation involved in mammalian gene activation is not completely defined. To identify additional sites of histone methylation that respond to mammalian gene activity, we describe here a comparative assessment of all six known positions of histone lysine methylation and relate them to gene transcription. Using several model loci, we observed high trimethylation of H3K4, H3K9, H3K36, and H3K79 in the transcribed region, consistent with previous findings. We identify H4K20 monomethylation, a modification previously linked with repression, as a mark of transcription elongation in mammalian cells. In contrast, H3K27 monomethylation, a modification enriched at pericentromeric heterochromatin, was observed broadly distributed throughout all eucliromatic sites analyzed, with selective depletion in the vicinity of the transcription start sites at active genes. Together, these results underscore that similar to other described methyl-lysine modifications, H4K20 and H3K27 monomethylation are versatile and dynamic with respect to gene activity, suggesting the existence of novel site-specific methyltransferases and demethylases coupled to the transcription cycle. Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Item Type: Paper
Uncontrolled Keywords: DNA histone histone lysine methyltransferase lysine methyl group site specific DNA methyltransferase (adenine specific) article cellular distribution controlled study DNA fingerprinting DNA methylation DNA modification DNA transcription euchromatin gene activation gene locus gene repression genome analysis heterochromatin human human cell mammal cell priority journal protein depletion protein function protein methylation protein modification transcription initiation site transcriptomics Chromatin Hela Cells Histones Humans Methylation Methyltransferases Poly(A)-Binding Protein I Transcription, Genetic Mammalia
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein methylation > histone methylation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein methylation
CSHL Authors:
Communities: CSHL labs > Vakoc lab
Depositing User: Matt Covey
Date Deposited: 13 Mar 2013 19:41
Last Modified: 13 Mar 2013 19:41
PMCID: PMC1698537
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27798

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