Sequences of bovine papillomavirus type 1 DNA--functional and evolutionary implications

Ahola, H., Stenlund, A., Moreno-Lopez, J., Pettersson, U. (May 1983) Sequences of bovine papillomavirus type 1 DNA--functional and evolutionary implications. Nucleic Acids Res, 11 (9). pp. 2639-50. ISSN 0305-1048 (Print)0305-1048 (Linking)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/6304656
DOI: 10.1093/nar/11.9.2639

Abstract

A swedish isolate of bovine papillomavirus type 1 (BPV1) was cloned and a 4807 base pairs long sequence was determined, covering the entire late region of the BPV1 genome and strategic parts of the early region. The sequence reveals two large uninterrupted reading frames, designated L1 and L2, which encode tentative polypeptides with molecular weights of 55.5 and 50.0 K. The promoter region for early transcription and the common 3'-end of early transcripts were identified in the sequence by performing S1 nuclease analysis of RNA isolated from a BPV1 transformed cell line. A detailed comparison between the established sequence and sequences from human papillomavirus type 1a (HPV1a) (1) and another BPV1 isolate (2) was also carried out. Only five differences were found when the sequences of the two BPV1 isolates were compared, two of which are located in reading frame L2. The results revealed furthermore that the genomes of BPV1 and HPV1a appear to be organized in a very similar fashion and the homology between reading frames L1 in BPV1 and HPV1 were particularly striking. A comparison of the established BPV1 sequence with sequences from SV40 and polyomavirus revealed no significant homology.

Item Type: Paper
Uncontrolled Keywords: Animals Base Sequence Biological Evolution Bovine papillomavirus 1/ genetics Cattle DNA, Viral/ genetics Gene Expression Regulation Humans Papillomaviridae/ genetics RNA, Viral/genetics Transcription, Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
organism description > virus
CSHL Authors:
Communities: CSHL labs > Stenlund lab
Depositing User: Matt Covey
Date: 11 May 1983
Date Deposited: 12 Mar 2013 18:46
Last Modified: 08 Nov 2017 17:11
PMCID: PMC325914
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27783

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