Introducing defined chromosomal rearrangements into the mouse genome

Zheng, B., Mills, A. A., Bradley, A. (2001) Introducing defined chromosomal rearrangements into the mouse genome. Methods, 24 (1). pp. 81-94. ISSN 10462023 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/11327806
DOI: 10.1006/meth.2001.1160

Abstract

Chromosomal rearrangements have been instrumental in genetic studies in Drosophila. Visibly marked deficiencies (deletions) are used in mapping studies and region-specific mutagenesis screens by providing segmental haploidy required to uncover recessive mutations. Marked recessive lethal inversions are used as balancer chromosomes to maintain recessive lethal mutations and to maintain the integrity of mutagenized chromosomes. In mice, studies on series of radiation-induced deletions that surround several visible mutations have yielded invaluable functional genomic information in the regions analyzed. However, most regions of the mouse genome are not accessible to such analyses due to a lack of marked chromosomal rearrangements. Here we describe a method to generate defined chromosomal rearrangements using the Cre-loxP recombination system based on a published strategy [R. Ramirez-Solis, P. Liu, and A. Bradley, (1995) Nature 378, 720-724]. Various types of rearrangements, such as deletions, duplications, inversions, and translocations, can be engineered using this strategy. Furthermore, the rearrangements can be visibly marked with coat color genes, providing essential reagents for large-scale recessive genetic screens in the mouse. The ability to generate marked chromosomal rearrangements will help to elevate the level of manipulative mouse genetics to that of Drosophila genetics. © 2001 Academic Press.

Item Type: Paper
Uncontrolled Keywords: cre recombinase gene product loxP protein unclassified drug article chromosome deletion chromosome duplication chromosome inversion chromosome rearrangement chromosome translocation Drosophila haploidy lethal mutant mouse mutagenesis nonhuman priority journal radiation Animals Blotting, Southern Chromosome Aberrations Genes, Recessive Genetic Markers Genetic Techniques Genetic Vectors In Situ Hybridization, Fluorescence Integrases Mice Models, Genetic Plasmids Polymerase Chain Reaction Recombination, Genetic Viral Proteins
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > genomes
organism description > animal > mammal > rodent > mouse
CSHL Authors:
Communities: CSHL labs > Mills lab
Depositing User: Matt Covey
Date: 2001
Date Deposited: 11 Mar 2013 20:19
Last Modified: 11 Mar 2013 20:19
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27757

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