From mouse to man: generating megabase chromosome rearrangements

Mills, A. A., Bradley, A. (June 2001) From mouse to man: generating megabase chromosome rearrangements. Trends in Genetics, 17 (6). pp. 331-339. ISSN 0168-9525

URL: http://www.ncbi.nlm.nih.gov/pubmed/11377795
DOI: 10.1016/S0168-9525(01)02321-6

Abstract

Experimental approaches for deciphering the function of human genes rely heavily on our ability to generate mutations in model organisms such as the mouse. However, because recessive mutations are masked by the wild-type allele in the diploid context, conventional mutagenesis and screening is often laborious and costly. Chromosome engineering combines the power of gene targeting in embryonic stem (ES) cells with Cre-loxP technology to create mice that are functionally haploid in discrete portions of the genome. Chromosome deletions, duplications and inversions can be tagged with visible markers, facilitating strain maintenance. These approaches allow for more refined mutagenesis screens that will greatly accelerate functional mouse genomics and generate mammalian models for developmental processes and cancer.

Item Type: Paper
Uncontrolled Keywords: EMBRYONIC STEM-CELLS DIGEORGE-SYNDROME REGION TRANSGENIC MICE RECOMBINATION MUTATIONS DELETIONS MUTAGENESIS FREQUENCY GENETICS VECTORS
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
organism description > animal > mammal > primates > hominids > human
organism description > animal > mammal > rodent > mouse
CSHL Authors:
Communities: CSHL labs > Mills lab
Depositing User: Matt Covey
Date: June 2001
Date Deposited: 11 Mar 2013 19:58
Last Modified: 11 Mar 2013 19:58
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27754

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