Changing colors in mice: an inducible system that delivers

Mills, A. A. (June 2001) Changing colors in mice: an inducible system that delivers. Genes & Development, 15 (12). pp. 1461-1467. ISSN 0890-9369

URL: http://www.ncbi.nlm.nih.gov/pubmed/11410526
DOI: 10.1101/gad.909301

Abstract

Expression of exogenous genes in mammalian systems can be extremely informative for deciphering the function of an encoded protein or determining the role of a putative regulatory region. In many instances it is desirable or even essential to employ a system that has the ability to be controlled by the experimenter. For example, embryonic lethality that results from expression of an exogenous transgene or disruption of an endogenous gene can be problematic as it precludes examination of the phenotypic consequence in the organism under study. In these cases the use of an inducible gene expression system would be advantageous so that gene expression could be turned on or off at will. Inducible systems would not only be useful for analyzing the function of exogenous genes, but may also be implemented in gene disruption experiments or in gene therapy protocols in which a therapeutic gene is delivered. Many laboratories have designed systems that aim to achieve inducible gene expression. However, even the best laid plans often yield disappointing results in vivo. This is attributable to an incomplete understanding of regulatory mechanisms that orchestrate mammalian gene expression. Exacerbating this problem is the fact that bacterial or viral genes and regulatory elements that are often implemented in the scheme may be rendered nonfunctional in mammalian systems, further decreasing the chance of success and increasing the chance of frustration.

Item Type: Paper
Uncontrolled Keywords: GENE-EXPRESSION TRANSGENIC MICE MAMMALIAN-CELLS REGULATED EXPRESSION LAC REPRESSOR GROWTH-FACTOR IN-VIVO RECEPTOR DIMERIZATION ACTIVATION
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
organism description > animal > mammal > rodent > mouse
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > transgenic animal
CSHL Authors:
Communities: CSHL labs > Mills lab
Depositing User: Matt Covey
Date: June 2001
Date Deposited: 11 Mar 2013 20:45
Last Modified: 11 Mar 2013 20:45
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27748

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