Differential expression of glutamate transporters EAAT1 and EAAT2 in mice deficient for PACAP-type I receptor

Zink, M., Schmitt, A., Henn, F. A., Gass, P. (December 2004) Differential expression of glutamate transporters EAAT1 and EAAT2 in mice deficient for PACAP-type I receptor. J Neural Transm, 111 (12). pp. 1537-42. ISSN 0300-9564 (Print)0300-9564 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/15565490
DOI: 10.1007/s00702-004-0175-2

Abstract

Pituitary adenylate cyclase-activating polypeptide (PACAP) modulates glutamatergic neurotransmission and induces the expression of glutamate transporters EAAT1 and EAAT2 in newborn mouse astroglial cell cultures. Since nanomolar concentrations of PACAP exert this effect, signal transduction via the high affinity PACAP-type I-receptor PAC1 was assumed. To test this hypothesis and to assess the importance of PAC1-signalling in vivo, we analyzed glutamate transporter expression in mice with a PAC1 knockout. EAAT1 and EAAT2 expression was investigated in the hippocampus and the cerebral cortex of PAC1 mutant mice and wildtype littermates by semiquantitative in-situ-hybridization. PAC1-knockout mice show a subtle but significant reduction of EAAT1 expression in the dentate gyrus. In contrast, reduced expression levels of EAAT1 in the cerebral cortex did not reach statistical significance and EAAT2 expression was unchanged in CA3 and cerebral cortex of PAC1 mutant mice. Our data confirm the previously reported in-vitro-regulation of EAAT1 in the adult nervous system in vivo. EAAT2 expression, however, is unchanged in PAC1 knockout mice, most likely due to counterbalancing factors.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Transport System X-AG/ biosynthesis Animals Autoradiography Brain/ metabolism Excitatory Amino Acid Transporter 1 Excitatory Amino Acid Transporter 2/ biosynthesis Glutamate Plasma Membrane Transport Proteins Image Processing, Computer-Assisted In Situ Hybridization Male Mice Mice, Knockout Receptors, Cell Surface/ deficiency Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I Symporters/ biosynthesis
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > glutamate receptor
organism description > animal > mammal > rodent > mouse
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
CSHL Authors:
Communities: CSHL labs > Henn lab
Depositing User: Matt Covey
Date: December 2004
Date Deposited: 04 Mar 2013 21:14
Last Modified: 04 Mar 2013 21:14
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27721

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