Analysis of transposon insertion mutants highlights the diversity of mechanisms underlying male progamic development in arabidopsis

Lalanne, E., Michaelidis, C., Moore, J. M., Gagliano, W., Johnson, A., Patel, R., Howden, R., Vielle-Calzada, J. P., Grossniklaus, U., Twell, D. (August 2004) Analysis of transposon insertion mutants highlights the diversity of mechanisms underlying male progamic development in arabidopsis. Genetics, 167 (4). pp. 1975-1986. ISSN 0016-6731

URL: http://www.ncbi.nlm.nih.gov/pubmed/15342534
DOI: 10.1534/genetics.104.030270

Abstract

To identify genes with essential roles in male gametophytic development, including postpollination (progamic) events, we have undertaken a genetic screen based on segregation ratio distortion of a transposon-borne kanamycin-resistance marker. In a population of 3359 Arabidopsis Ds transposon insertion lines, we identified 20 mutants with stably reduced segregation ratios arising from reduced gametophytic transmission. All 20 mutants showed strict cosegregation of Ds and the reduced gametophytic transmission phenotype. Among these, 10 mutants affected both male and female transmission and 10 mutants showed male-specific transmission defects. Four male and female (ungud) mutants and 1 male-specific mutant showed cellular defects in microspores and/or in developing pollen. The 6 remaining ungud mutants and 9 male-specific (seth) mutants affected pollen functions during progamic development. In vitro and in vivo analyses are reported for 5 seth mutants. seth6 completely blocked pollen germination, while seth7 strongly reduced pollen germination efficiency and tube growth. In contrast, seth8, seth9, or seth10 pollen showed reduced competitive ability that was linked to slower rates of pollen tube growth. Gene sequences disrupted in seth insertions suggest essential functions for Putative SETH proteins in diverse processes including protein anchoring, cell wall biosynthesis, signaling, and metabolism.

Item Type: Paper
Uncontrolled Keywords: POLLEN-TUBE GROWTH SEGREGATION DISTORTION GIBBERELLIN 2-OXIDASES GAMETOPHYTIC MUTANTS CELL-FATE THALIANA PROTEIN FEMALE GENE GUIDANCE
Subjects: organism description > plant > Arabidopsis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
organism description > plant
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transposons
CSHL Authors:
Communities: CSHL labs > Grossniklaus lab
Depositing User: Matt Covey
Date: August 2004
Date Deposited: 04 Mar 2013 20:49
Last Modified: 04 Mar 2013 20:49
PMCID: PMC1471024
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27711

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