Reduced expression of brain-derived neurotrophic factor in mice deficient for pituitary adenylate cyclase activating polypeptide type-I-receptor

Zink, M., Otto, C., Zorner, B., Zacher, C., Schutz, G., Henn, F. A., Gass, P. (April 2004) Reduced expression of brain-derived neurotrophic factor in mice deficient for pituitary adenylate cyclase activating polypeptide type-I-receptor. Neurosci Lett, 360 (1-2). pp. 106-8. ISSN 0304-3940 (Print)0304-3940 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/15082190
DOI: 10.1016/j.neulet.2004.01.030

Abstract

In vitro pituitary adenylate cyclase activating polypeptide (PACAP) induces the expression of brain-derived neurotrophic factor (BDNF) via its specific receptor PAC1. Since BDNF has been implicated in learning paradigms and mice lacking functional PAC1 have deficits in hippocampus-dependent associative learning, we investigated whether PAC1 mutants show alterations in hippocampal expression of BDNF and its receptor TrkB. Semi-quantitative in situ-hybridization using exon-specific BDNF-probes revealed significantly reduced expression of the exon-III and exon-V-specific transcripts within the hippocampal CA3 region in PAC1-deficient mice. A similar trend was observed for the exon-I-specific transcript. The expression of the exon-III-specific transcript was also reduced within the dentate gyrus, while Trk B-expression did not differ between genotypes. Our data demonstrate that even in vivo PAC1-mediated signaling seems to play a pivotal role for the transcriptional regulation of BDNF.

Item Type: Paper
Uncontrolled Keywords: Animals Brain-Derived Neurotrophic Factor/ biosynthesis/genetics Gene Expression Regulation/physiology Hippocampus/metabolism Male Mice Mice, Inbred C57BL Mice, Transgenic Pilot Projects Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I Receptors, Pituitary Hormone/ deficiency/genetics
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
organism description > animal > mammal > rodent > mouse
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
CSHL Authors:
Communities: CSHL labs > Henn lab
Depositing User: Matt Covey
Date: 22 April 2004
Date Deposited: 04 Mar 2013 21:34
Last Modified: 04 Mar 2013 21:34
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27701

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