RNA polymerase II is required for RNAi-dependent heterochromatin assembly

Kato, H., Goto, D. B., Martienssen, R. A., Urano, T., Furukawa, K., Murakami, Y. (2005) RNA polymerase II is required for RNAi-dependent heterochromatin assembly. Science, 309 (5733). pp. 467-469. ISSN 00368075 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/15947136
DOI: 10.1126/science.1114955

Abstract

In Schizosaccharomyces pombe, the RNA interference (RNAi) machinery converts pericentromeric transcripts into small interfering RNAs (siRNAs) and is required for the assembly of pericentromeric heterochromatin. Here we describe a mutation in the second largest subunit of RNA polymerase II (RNAPII). Both wild-type and mutant RNAPII localized to the pericentromere. However, the mutation resulted in the loss of heterochromatic histone modifications and in the accumulation of pericentromeric transcripts, accompanied by the loss of siRNAs. This phenotype resembles mutants in RNAi and suggests that RNAPII couples pericentromeric transcription with siRNA processing and heterochromatin assembly.

Item Type: Paper
Uncontrolled Keywords: Polymers RNA Surface treatment Pericentromeric transcription RNA interference (RNAi) RNA polymerase II (RNAPII) Small interfering RNAs (siRNA) Molecular biology histone RNA polymerase II small interfering RNA article centromere enzyme localization enzyme subunit heterochromatin phenotype priority journal protein modification RNA interference RNA transcription Schizosaccharomyces pombe wild type
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > RNA polymerase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
CSHL Authors:
Communities: CSHL labs > Martienssen lab
Depositing User: Matt Covey
Date: 2005
Date Deposited: 01 Mar 2013 21:50
Last Modified: 01 Mar 2013 21:50
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27675

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