CHR11, a chromatin-remodeling factor essential for nuclear proliferation during female gametogenesis in Arabidopsis thaliana

Huanca-Mamani, W., Garcia-Aguilar, M., Leon-Martinez, G., Grossniklaus, U., Vielle-Calzada, J. P. (November 2005) CHR11, a chromatin-remodeling factor essential for nuclear proliferation during female gametogenesis in Arabidopsis thaliana. Proc Natl Acad Sci U S A, 102 (47). pp. 17231-17236. ISSN 0027-8424

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URL: http://www.ncbi.nlm.nih.gov/pubmed/16286646
DOI: 10.1073/pnas.0508186102

Abstract

Chromatin-remodeling factors regulate the establishment of transcriptional programs during plant development. Although 42 genes encoding members of the SWI2/SNF2 family have been identified in Arabidopsis thaliana, < 10 have been assigned a precise function on the basis of a mutant phenotype, and none have been shown to play a specific role during the gametophytic phase of the plant life cycle. A. thaliana chromatin-remodeling protein 11 (CHR11) encodes an imitation of switch (ISWI)-like chromatin-remodeling protein abundantly expressed during female gametogenesis and embryogenesis in Arabidopsis. To determine the function of CHR11 in wild-type plants, we introduced a hairpin construct leading to the production of double-stranded RNA, which specifically degraded the endogenous CHR11 mRNA by RNA interference (RNAi). Transcription of the RNAi-inducing hairpin RNA was driven by either a constitutive cauliflower mosaic virus 35S promoter (CaMV35S) acting at most stages of the sporophytic phase or a newly identified specific promoter acting at the onset of the female gametophytic phase (pFM1). All adult trans-formants that constitutively lacked sporophytic CHR11 activity showed reduced plant height and small cotyledonary embryos with limited cell expansion. In contrast, RNAi lines in which CHR11 was specifically silenced at the onset of female gametogenesis (megagametogenesis) had normal height and embryo size but had defective female gametophytes arrested before the completion of the mitotic haploid nuclear divisions. These results show that CHR11 is essential for haploid nuclear proliferation during megagametogenesis and cell expansion during the sporophytic phase, demonstrating the functional versatility of SW12/SNF2 chromatin-remodeling factors during both generations of the plant life cycle.

Item Type: Paper
Uncontrolled Keywords: imitation of switch proteins megagametophyte RNA interference seed development functional megaspore TRANSCRIPTION FACTOR PLANT DEVELOPMENT DNA METHYLATION SANT DOMAIN N-COR GENE RNA PROTEIN COMPLEX ENDOREDUPLICATION
Subjects: organism description > plant > Arabidopsis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics
organism description > plant
CSHL Authors:
Communities: CSHL labs > Grossniklaus lab
Depositing User: Matt Covey
Date: November 2005
Date Deposited: 01 Mar 2013 21:58
Last Modified: 09 Nov 2017 16:19
PMCID: PMC1287998
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27664

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