Reduced expression of complexins I and II in rats bred for learned helplessness

Zink, M., Vollmayr, B., Gebicke-Haerter, P. J., Henn, F. A., Thome, J. (May 2007) Reduced expression of complexins I and II in rats bred for learned helplessness. Brain Res, 1144. pp. 202-8. ISSN 0006-8993 (Print)0006-8993 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/17320830
DOI: 10.1016/j.brainres.2007.01.066

Abstract

Disturbed synaptic transmission contributes to the pathophysiology of mood disorders. Post mortem studies reported reduced expression of the synaptic vesicle protein (SVP) complexins I and II in depression. Antidepressants were found to induce the expression of these genes. Since animals with congenital susceptibility to learned helplessness provide a valid animal model of depression, we investigated the expression of different SVPs in this system by semiquantitative in situ hybridization. Rats bred for congenital learned helpless behavior (cLH, N=6) failed to interrupt foot shock currents by lever pressing (mean 12.3 failures out of 15 trials). These animals showed significantly lower expression of complexins I and II mRNA in hippocampal, limbic and cortical brain areas compared to not helpless animals (cNLH, N=6) with a mean failure rate of 0.83 out of 15 trials. Expression levels of complexins I and II significantly correlated with the failure rate in the test paradigm. In contrast, the expressions of synaptotagmin I and synaptophysin were found unchanged. This investigation provides a further validation of the LH model of depression. The experimental data fit well into current pathogenetic concepts of mood disorders and support the hypothesis, that complexins are pivotal players in the pathophysiology of depression and tentative targets of antidepressants.

Item Type: Paper
Uncontrolled Keywords: Adaptor Proteins, Vesicular Transport Animals Autoradiography Brain/anatomy & histology/ metabolism Escape Reaction/physiology Gene Expression Regulation/ physiology Helplessness, Learned In Situ Hybridization/methods Male Nerve Tissue Proteins/ metabolism Rats Rats, Sprague-Dawley Rats, Wistar Statistics, Nonparametric Synaptophysin/metabolism Synaptotagmin I/metabolism
Subjects: diseases & disorders
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
diseases & disorders > mental disorders
diseases & disorders > mental disorders > mood disorders
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
organism description > animal
diseases & disorders > mental disorders > mood disorders > depression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
organism description > animal > mammal > rodent > rat
organism description > animal > mammal > rodent > rat
CSHL Authors:
Communities: CSHL labs > Henn lab
Depositing User: Matt Covey
Date: 4 May 2007
Date Deposited: 28 Feb 2013 21:23
Last Modified: 28 Feb 2013 21:23
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27650

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