A single Hox locus in Drosophila produces functional microRNAs from opposite DNA strands

Stark, A., Bushati, N., Jan, C. H., Kheradpour, P., Hodges, E., Brennecke, J., Bartel, D. P., Cohen, S. M., Kellis, M. (January 2008) A single Hox locus in Drosophila produces functional microRNAs from opposite DNA strands. Genes Dev, 22 (1). pp. 8-13.

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URL: http://www.ncbi.nlm.nih.gov/pubmed/18172160
DOI: 10.1101/gad.1613108

Abstract

MicroRNAs (miRNAs) are approximately 22-nucleotide RNAs that are processed from characteristic precursor hairpins and pair to sites in messages of protein-coding genes to direct post-transcriptional repression. Here, we report that the miRNA iab-4 locus in the Drosophila Hox cluster is transcribed convergently from both DNA strands, giving rise to two distinct functional miRNAs. Both sense and antisense miRNA products target neighboring Hox genes via highly conserved sites, leading to homeotic transformations when ectopically expressed. We also report sense/antisense miRNAs in mouse and find antisense transcripts close to many miRNAs in both flies and mammals, suggesting that additional sense/antisense pairs exist.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
organism description > animal > insect > Drosophila
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
organism description > animal
organism description > animal > insect
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA
CSHL Authors:
Communities: CSHL labs > Hannon lab
Depositing User: Matt Covey
Date: 1 January 2008
Date Deposited: 25 Feb 2013 20:28
Last Modified: 03 Nov 2017 20:21
PMCID: PMC2151017
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27621

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