RNA interference in mammalian cell systems

Paddison, P. J. (2008) RNA interference in mammalian cell systems. In: RNA Interference. Curr Top Microbiol Immunol, 320 . Springer Verlag, Berlin; New York, pp. 1-19. ISBN 978-3-540-75156-4

URL: http://www.ncbi.nlm.nih.gov/pubmed/18268837
DOI: 10.1007/978-3-540-75157-1_1

Abstract

The use of RNA interference (RNAi) to evoke gene silencing in mammalian cells has almost become routine laboratory practice. Through refinement of double-stranded RNA (dsRNA) triggers of RNAi and creation of genome-scale libraries, the first genome-wide loss of function screens have been carried out in mammals. This review discusses some of the key features of RNAi in mammalian systems.

Item Type: Book Section
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > design
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
Investigative techniques and equipment
bioinformatics > genomics and proteomics > design > nucleic acid design
Investigative techniques and equipment > RNAI
bioinformatics > genomics and proteomics > design > nucleic acid design > RNAI Design
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi
CSHL Authors:
Communities: CSHL labs > Paddison lab
Depositing User: Matt Covey
Date: 2008
Date Deposited: 25 Feb 2013 17:18
Last Modified: 25 Feb 2013 17:18
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27615

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