RanGAP1 is a continuous marker of the Arabidopsis cell division plane

Xu, X. M., Zhao, Q., Rodrigo-Peiris, T., Brkljacic, J., He, C. S., Muller, S., Meier, I. (November 2008) RanGAP1 is a continuous marker of the Arabidopsis cell division plane. Proc Natl Acad Sci U S A, 105 (47). pp. 18637-18642.

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URL: http://www.ncbi.nlm.nih.gov/pubmed/19011093
DOI: 10.1073/pnas.0806157105

Abstract

In higher plants, the plane of cell division is faithfully predicted by the preprophase band (PPB). The PPB, a cortical ring of microtubules and F-actin, disassembles upon nuclear-envelope breakdown. During cytokinesis, the expanding cell plate fuses with the plasma membrane at the cortical division site, the site of the former PPB. The nature of the “molecular memory� that is left behind by the PPB and is proposed to guide the cell plate to the cortical division site is unknown. RanGAP is the GTPase activating protein of the small GTPase Ran, which provides spatial information for nucleocytoplasmic transport and various mitotic processes in animals. Here, we show that, in dividing root cells, RanGAP1 concentrates at the PPB and remains associated with the cortical division site during mitosis and cytokinesis, requiring its N-terminal targeting domain. In a / mutant, which affects PPB formation, RanGAP1 recruitment to the PPB site is lost, while its PPB retention is microtubule-independent. RanGAP1 persistence at the cortical division site, but not its initial accumulation at the PPB requires the 2 cytokinesis-regulating kinesins POK1 and POK2. Depletion of RanGAP by inducible RNAi leads to oblique cell walls and cell-wall stubs in root cell files, consistent with cytokinesis defects. We propose that RanGAP, a continuous positive protein marker of the plant division plane, has a role in spatial signaling during plant cell division.

Item Type: Paper
Uncontrolled Keywords: cytokinesis Ran cycle preprophase band nuclear pore
Subjects: organism description > plant > Arabidopsis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
organism description > plant
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
CSHL Authors:
Communities: CSHL labs > Lowe lab
Depositing User: Matt Covey
Date: 25 November 2008
Date Deposited: 25 Feb 2013 21:53
Last Modified: 08 Nov 2017 21:00
PMCID: PMC2587610
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27600

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