Electron transfer dissociation of iTRAQ labeled peptide ions

Han, H., Pappin, D. J., Ross, P. L., McLuckey, S. A. (September 2008) Electron transfer dissociation of iTRAQ labeled peptide ions. J Proteome Res, 7 (9). pp. 3643-8. ISSN 1535-3893 (Print)1535-3893 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/18646790
DOI: 10.1021/pr8001113

Abstract

Triply and doubly charged iTRAQ ( isobaric tagging for relative and absolute quantitation) labeled peptide cations from a tryptic peptide mixture of bovine carbonic anhydrase II were subjected to electron transfer ion/ion reactions to investigate the effect of charge bearing modifications associated with iTRAQ on the fragmentation pattern. It was noted that electron transfer dissociation (ETD) of triply charged or activated ETD (ETD and supplemental collisional activation of intact electron transfer species) of doubly charged iTRAQ tagged peptide ions yielded extensive sequence information, in analogy with ETD of unmodified peptide ions. That is, addition of the fixed charge iTRAQ tag showed relatively little deleterious effect on the ETD performance of the modified peptides. ETD of the triply charged iTRAQ labeled peptide ions followed by collision-induced dissociation (CID) of the product ion at m/ z 162 yielded the reporter ion at m/ z 116, which is the reporter ion used for quantitation via CID of the same precursor ions. The reporter ion formed via the two-step activation process is expected to provide quantitative information similar to that directly produced from CID. A 103 Da neutral loss species observed in the ETD spectra of all the triply and doubly charged iTRAQ labeled peptide ions is unique to the 116 Da iTRAQ reagent, which implies that this process also has potential for quantitation of peptides/proteins. Therefore, ETD with or without supplemental collisional activation, depending on the precursor ion charge state, has the potential to directly identify and quantify the peptides/proteins simultaneously using existing iTRAQ reagents.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Electron Transport Peptide Mapping Peptides/ chemistry Tandem Mass Spectrometry Trypsin/chemistry
Subjects: bioinformatics > genomics and proteomics
Investigative techniques and equipment
CSHL Authors:
Communities: CSHL labs > Pappin lab
Depositing User: Matt Covey
Date: September 2008
Date Deposited: 26 Feb 2013 21:06
Last Modified: 26 Feb 2013 21:06
PMCID: 2668817
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27518

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