Dicer is required for maintenance of hair follicle stem cells in adult skin

Teta, M., Thomas, A., Okegbe, T., Murchison, E. P., Nagy, A., Hannon, G. J., Millar, S. E. (July 2008) Dicer is required for maintenance of hair follicle stem cells in adult skin. Developmental Biology, 319 (2). p. 556. ISSN 0012-1606

URL: http://www.sciencedirect.com/science/article/pii/S...
DOI: 10.1016/j.ydbio.2008.05.316

Abstract

Multiple miRNAs are expressed in developing and postnatal skin and hair follicles, and constitutive deletion of the miRNA processing enzyme Dicer in embryonic mouse skin causes failure of hair follicle morphogenesis and subsequent follicular degradation. To determine whether Dicer and miRNA functions are required in established hair follicles and epidermis in the adult mouse we generated Krt5-rtTA tetO-Cre Dicerfl/fl mice in which the Dicer gene can be inducibly deleted in the basal epidermis and hair follicle outer root sheath, including hair follicle stem cells, by dosage with oral doxycycline. Inducible Dicer deletion starting at postnatal day (P) 20, when hair follicles are just entering the first postnatal growth cycle, resulted in loss of external hair starting within 10days of doxycycline treatment. Induction of mutation during the resting stage of the hair follicle growth cycle did not immediately result in histological abnormalities or loss of hair. However, expression of the hair follicle bulge stem cell markers K15 and CD34 was lost soon after induction. Subsequent hair plucking stimulated a new cycle of hair follicle growth in control littermate, but not induced Dicer mutant skin. These results demonstrate a requirement for Dicer in maintaining the ability of adult hair follicles to grow and regenerate, and indicate that Dicer is required for maintenance of hair follicle epithelial stem cells.

Item Type: Paper
Additional Information: Meeting Abstract
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
organism description > animal > developmental stage > adult
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > dicer
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > dicer

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes
Publication Type > Meeting Abstract
organs, tissues, organelles, cell types and functions > organs types and functions
organs, tissues, organelles, cell types and functions
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
organs, tissues, organelles, cell types and functions > organs types and functions > skin
therapies > stem cells
CSHL Authors:
Communities: CSHL labs > Hannon lab
Watson School > Publications
Depositing User: Matt Covey
Date: 15 July 2008
Date Deposited: 26 Feb 2013 21:35
Last Modified: 03 Jan 2019 21:35
URI: http://repository.cshl.edu/id/eprint/27514

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