ABAP1 is a novel plant Armadillo BTB protein involved in DNA replication and transcription

Masuda, H. P., Cabral, L. M., De Veylder, L., Tanurdzic, M., de Almeida Engler, J., Geelen, D., Inze, D., Martienssen, R. A., Ferreira, P. C., Hemerly, A. S. (October 2008) ABAP1 is a novel plant Armadillo BTB protein involved in DNA replication and transcription. EMBO Journal, 27 (20). pp. 2746-56. ISSN 1460-2075 (Electronic)0261-4189 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/18818695
DOI: 10.1038/emboj.2008.191

Abstract

In multicellular organisms, organogenesis requires a tight control of the balance between cell division and cell differentiation. Distinct signalling pathways that connect both cellular processes with developmental cues might have evolved to suit different developmental plans. Here, we identified and characterized a novel protein that interacts with pre-replication complex (pre-RC) subunits, designated Armadillo BTB Arabidopsis protein 1 (ABAP1). Overexpression of ABAP1 in plants limited mitotic DNA replication and decreased cell proliferation in leaves, whereas ABAP1 downregulation increased cell division rates. Activity of ABAP1 in transcription was supported by its association with the transcription factor AtTCP24. The ABAP1-AtTCP24 complex bound specifically to the promoters of AtCDT1a and AtCDT1b in vitro and in vivo. Moreover, expression levels of AtCDT1a and AtCDT1b were reduced in ABAP1-overexpressing plants and they were increased in plants with reduced levels of ABAP1. We propose that ABAP1 participates in a negative feedback loop regulating mitotic DNA replication during leaf development, either by repressing transcription of pre-RC genes and possibly by regulating pre-RC utilization through direct association with pre-RC components.

Item Type: Paper
Uncontrolled Keywords: Arabidopsis/*metabolism Arabidopsis Proteins/genetics/*metabolism Armadillo Domain Proteins/metabolism Cell Division Cell Proliferation DNA/metabolism DNA Replication Down-Regulation Genes, Plant Mitosis Models, Biological Nuclear Proteins/genetics/metabolism Plant Leaves/metabolism Protein Binding Signal Transduction Transcription Factors/genetics/*metabolism *Transcription, Genetic Two-Hybrid System Techniques
Subjects: organism description > plant > Arabidopsis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
organism description > plant
CSHL Authors:
Communities: CSHL labs > Martienssen lab
Depositing User: Matt Covey
Date: 22 October 2008
Date Deposited: 28 Feb 2013 17:01
Last Modified: 09 Apr 2014 19:53
PMCID: PMC2572174
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27484

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