The transcriptome of human CD34+ hematopoietic stem-progenitor cells

Kim, Y. C., Wu, Q., Chen, J., Xuan, Z., Jung, Y.-C., Zhang, M. Q., Rowley, J. D., Wang, S. M. (May 2009) The transcriptome of human CD34+ hematopoietic stem-progenitor cells. Proc Natl Acad Sci U S A, 106 (20). pp. 8278-8283.

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URL: http://www.ncbi.nlm.nih.gov/pubmed/19416867
DOI: 10.1073/pnas.0903390106

Abstract

Studying gene expression at different hematopoietic stages provides insights for understanding the genetic basis of hematopoiesis. We analyzed gene expression in human CD34 hematopoietic cells that represent the stem-progenitor population (CD34 cells). We collected >459,000 transcript signatures from CD34 cells, including the de novo-generated 3′ ESTs and the existing sequences of full-length cDNAs, ESTs, and serial analysis of gene expression (SAGE) tags, and performed an extensive annotation on this large set of CD34 transcript sequences. We determined the genes expressed in CD34 cells, verified the known genes and identified the new genes of different functional categories involved in hematopoiesis, dissected the alternative gene expression including alternative transcription initiation, splicing, and adenylation, identified the antisense and noncoding transcripts, determined the CD34 cell-specific gene expression signature, and developed the CD34 cell-transcription map in the human genome. Our study provides a current view on gene expression in human CD34 cells and reveals that early hematopoiesis is an orchestrated process with the involvement of over half of the human genes distributed in various functions. The data generated from our study provide a comprehensive and uniform resource for studying hematopoiesis and stem cell biology.

Item Type: Paper
Uncontrolled Keywords: gene expression genome map annotation hemotopoiesis stem cell
Subjects: bioinformatics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
organs, tissues, organelles, cell types and functions > cell types and functions > cell types
organs, tissues, organelles, cell types and functions > cell types and functions > cell types
organs, tissues, organelles, cell types and functions > cell types and functions > cell types

organs, tissues, organelles, cell types and functions > cell types and functions > cell types > progenitor cell
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > progenitor cell
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > progenitor cell

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > transcriptomes
CSHL Authors:
Communities: CSHL labs > Zhang lab
CSHL Cancer Center Shared Resources > Bioinformatics Service
Depositing User: Matt Covey
Date: 19 May 2009
Date Deposited: 15 Feb 2013 20:28
Last Modified: 08 Nov 2017 20:49
PMCID: PMC2688877
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27471

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