Laser microdissection-mediated isolation and in vitro transcriptional amplification of plant RNA

Scanlon, M. J., Ohtsu, K., Timmermans, M. C. P., Schnable, P. S. (July 2009) Laser microdissection-mediated isolation and in vitro transcriptional amplification of plant RNA. Current Protocols in Molecular Biology (SUPPL.). 25A.3.1-25A.3.15.

URL: http://www.ncbi.nlm.nih.gov/pubmed/19575479
DOI: 10.1002/0471142727.mb25a03s87

Abstract

Protocols for laser microdissection and linear amplification of RNA from fixed, sectioned plant tissues are described. When combined with quantitative RT-PCR, microarray analysis, or RNA-sequencing, these procedures enable quantitative analyses of transcript accumulation from microscopic quantities of specific plant organs, tissues, or single cells. © 2009 by John Wiley & Sons, Inc.

Item Type: Paper
Uncontrolled Keywords: Laser microdissection Plants RNA amplification Transcriptomics
Subjects: bioinformatics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
Investigative techniques and equipment
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA expression
organism description > plant
CSHL Authors:
Communities: CSHL labs > Timmermans lab
Depositing User: Matt Covey
Date: July 2009
Date Deposited: 20 Feb 2013 22:06
Last Modified: 20 Feb 2013 22:06
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27403

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