Gal4 turnover and transcription activation

Collins, G. A., Lipford, J. R., Deshaies, R. J., Tansey, W. P. (2009) Gal4 turnover and transcription activation. Nature, 461 (7265). E7-E7.

URL: http://www.ncbi.nlm.nih.gov/pubmed/19812621
DOI: 10.1038/nature08406

Abstract

Growing evidence supports the notion that proteasome-mediated destruction of transcriptional activators can be intimately coupled to their function. Recently, Nalley et al. challenged this view by reporting that the prototypical yeast activator Gal4 does not dynamically associate with chromatin, but rather 'locks in' to stable promoter complexes that are resistant to competition. Here we present evidence that the assay used to reach this conclusion is unsuitable, and that promoter-bound, active Gal4 is indeed susceptible to competition in vivo. Our data challenge the key evidence that Nalley et al. used to reach their conclusion, and indicate that Gal4 functions in vivo within the context of dynamic promoter complexes.

Item Type: Paper
Subjects: bioinformatics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
CSHL Authors:
Communities: CSHL labs > Tansey lab
Watson School > Publications
Depositing User: Matt Covey
Date Deposited: 21 Feb 2013 14:26
Last Modified: 22 Sep 2014 18:26
PMCID: PMC3072683
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27383

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