Fluorescent Arc/Arg3.1 indicator mice: A versatile tool to study brain activity changes in vitro and in vivo

Grinevich, V., Kolleker, A., Eliava, M., Takada, N., Takuma, H., Fukazawa, Y., Shigemoto, R., Kuhl, D., Waters, J., Seeburg, P.H., Osten, P. (October 2009) Fluorescent Arc/Arg3.1 indicator mice: A versatile tool to study brain activity changes in vitro and in vivo. Journal of Neuroscience Methods, 184 (1). pp. 25-36.

URL: http://www.ncbi.nlm.nih.gov/pubmed/19628007
DOI: 10.1016/j.jneumeth.2009.07.015

Abstract

The brain-specific immediate early gene Arc/Arg3.1 is induced in response to a variety of stimuli, including sensory and behavior-linked neural activity. Here we report the generation of transgenic mice, termed TgArc/Arg3.1-d4EGFP, expressing a 4-h half-life form of enhanced green fluorescent protein (d4EGFP) under the control of the Arc/Arg3.1 promoter. We show that d4EGFP-mediated fluorescence faithfully reports Arc/Arg3.1 induction in response to physiological, pathological and pharmacological stimuli, and that this fluorescence permits electrical recording from activated neurons in the live mouse. Moreover, the fluorescent Arc/Arg3.1 indicator revealed activity changes in circumscribed brain areas in distinct modes of stress and in a mouse model of Alzheimer's disease. These findings identify the TgArc/Arg3.1-d4EGFP mouse as a versatile tool to monitor Arc/Arg3.1 induction in neural circuits, both in vitro and in vivo.

Item Type: Paper
Uncontrolled Keywords: Immediate early genes Arc Arg3.1 Green fluorescent protein (GFP) Transgenic mice Synaptic plasticity Neural circuits Stress
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
organism description > animal
organs, tissues, organelles, cell types and functions > organs types and functions > brain
organism description > animal > mammal > rodent > mouse
organs, tissues, organelles, cell types and functions > organs types and functions
organs, tissues, organelles, cell types and functions
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > transgenic animal
CSHL Authors:
Communities: CSHL labs > Osten lab
CSHL Post Doctoral Fellows
Depositing User: Matt Covey
Date: October 2009
Date Deposited: 21 Feb 2013 17:00
Last Modified: 03 May 2013 14:59
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27380

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