Extensive conservation of genomic imbalances in canine transmissible venereal tumors (CTVT) detected by microarray-based CGH analysis

Thomas, R., Rebbeck, C. A., Leroi, A. M., Burt, A., Breen, M. (October 2009) Extensive conservation of genomic imbalances in canine transmissible venereal tumors (CTVT) detected by microarray-based CGH analysis. Chromosome Research, 17 (7). pp. 927-934. ISSN 0967-3849

URL: http://www.ncbi.nlm.nih.gov/pubmed/19798471
DOI: 10.1007/s10577-009-9080-8

Abstract

Canine transmissible venereal tumor (CTVT) is an intriguing cancer that is transmitted naturally as an allograft by transplantation of viable tumor cells from affected to susceptible dogs. At least initially, the tumor is able to evade the host's immune response; thus, CTVT has potential to provide novel insights into tumor immunobiology. The nature of CTVT as a "contagious" cancer, originating from a common ancestral source of infection, has been demonstrated previously by a series of studies comparing geographically distinct tumors at the molecular level. While these studies have revealed that apparently unrelated tumors share a striking degree of karyotypic conservation, technological restraints have limited the ability to investigate the chromosome composition of CTVTs in any detail. We present characterization of a strategically selected panel of CTVT cases using microarray-based comparative genomic hybridization analysis at ~one-megabase resolution. These data show for the first time that the tumor presents with an extensive range of non-random chromosome copy number aberrations that are distributed widely throughout the dog genome. The majority of abnormalities detected were imbalances of small subchromosomal regions, often involving centromeric and telomeric sequences. All cases also showed the sex chromosome complement XO. There was remarkable conservation in the cytogenetic profiles of the tumors analyzed, with only minor variation observed between different cases. These data suggest that the CTVT genome demonstrates a vast degree of both structural and numerical reorganization that is maintained during transmission among the domestic dog population. © 2009 Springer Science+Business Media B.V.

Item Type: Paper
Uncontrolled Keywords: comparative genomic hybridization (CGH) canine transmissible venereal tumor chromosome microarray BAC MICROARRAY CLONAL ORIGIN DOG CHROMOSOME CANCER IDENTIFICATION CONSTRUCTION KARYOTYPE EVOLUTION SEQUENCE
Subjects: bioinformatics > genomics and proteomics > analysis and processing
bioinformatics
diseases & disorders > cancer
diseases & disorders
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > analysis and processing > microarray gene expression processing
CSHL Authors:
Communities: CSHL labs > Hannon lab
CSHL Post Doctoral Fellows
Depositing User: Matt Covey
Date: October 2009
Date Deposited: 21 Feb 2013 16:41
Last Modified: 03 May 2013 14:01
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27374

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