AMPA Receptor Incorporation into Synapses during LTP: The Role of Lateral Movement and Exocytosis

Makino, H., Malinow, R. (November 2009) AMPA Receptor Incorporation into Synapses during LTP: The Role of Lateral Movement and Exocytosis. Neuron, 64 (3). pp. 381-390. ISSN 0896-6273

URL: http://www.ncbi.nlm.nih.gov/pubmed/19914186
DOI: 10.1016/j.neuron.2009.08.035

Abstract

The regulated trafficking of AMPA receptors (AMPARs) to synapses is thought to underlie the enhanced transmission in long-term potentiation (LTP), a cellular model of memory. However, there is controversy regarding the nonsynaptic site, either on the surface or intracellularly, from which AMPARs move into synapses during LTP. Using recombinant surface-fluorescent receptors in organotypic rat hippocampal slices, we show that the majority of AMPARs incorporated into synapses during LTP is from lateral diffusion of spine surface receptors containing GluR1, an AMPAR subunit. Following synaptic potentiation, AMPARs in intracellular pools containing GluR1 are driven to the surface primarily on dendrites. These exocytosed receptors likely serve to replenish the local extrasynaptic pool available for subsequent bouts of plasticity. These results clarify the role of intracellular and surface AMPARs during synaptic plasticity.

Item Type: Paper
Uncontrolled Keywords: LONG-TERM POTENTIATION SYNAPTIC PLASTICITY DENDRITIC SPINES RECYCLING ENDOSOMES POSTSYNAPTIC MEMBRANE EXCITATORY SYNAPSES PYRAMIDAL NEURONS TRAFFICKING GLUR1 DELIVERY
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > AMPA receptor
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
organs, tissues, organelles, cell types and functions
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions
organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > synapse
CSHL Authors:
Communities: CSHL labs > Malinow lab
Watson School > Publications
Depositing User: Matt Covey
Date: 12 November 2009
Date Deposited: 21 Feb 2013 21:26
Last Modified: 22 Sep 2014 18:31
PMCID: PMC2999463
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27334

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