A Protodermal miR394 Signal Defines a Region of Stem Cell Competence in the Arabidopsis Shoot Meristem

Knauer, S., Holt, A.L., Rubio-Somoza, I., Tucker, E. J., Hinze, A., Pisch, M., Javelle, M., Timmermans, M. C. P., Tucker, M. R., Laux, T. (January 2013) A Protodermal miR394 Signal Defines a Region of Stem Cell Competence in the Arabidopsis Shoot Meristem. Developmental Cell. ISSN 15345807

URL: http://www.ncbi.nlm.nih.gov/pubmed/23333352
DOI: 10.1016/j.devcel.2012.12.009

Abstract

A long-standing question in plants and animals is how spatial patterns are maintained within stem cell niches despite ongoing cell divisions. Here we address how, during shoot meristem formation in Arabidopsis thaliana, the three apical cell layers acquire stem cell identity. Using a sensitized mutant screen, we identified miR394 as a mobile signal produced by the surface cell layer (the protoderm) that confers stem cell competence to the distal meristem by repressing the F box protein LEAF CURLING RESPONSIVENESS. This repression is required to potentiate signaling from underneath the stem cells by the transcription factor WUSCHEL, maintaining stem cell pluripotency. The interaction of two opposing signaling centers provides a mechanistic framework of how stem cells are localized at the tip of the meristem. Although the constituent cells change, the surface layer provides a stable point of reference in the self-organizing meristem. Copyright © 2013 Elsevier Inc. All rights reserved.

Item Type: Paper
Subjects: organism description > plant > Arabidopsis
bioinformatics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA

organs, tissues, organelles, cell types and functions > tissues types and functions > meristem
organs, tissues, organelles, cell types and functions
organism description > plant
organs, tissues, organelles, cell types and functions > tissues types and functions
CSHL Authors:
Communities: CSHL Post Doctoral Fellows
CSHL labs > Timmermans lab
Depositing User: Matt Covey
Date: January 2013
Date Deposited: 11 Feb 2013 16:19
Last Modified: 17 Feb 2017 16:25
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27235

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