Phosphorylation of H4 Ser 47 promotes HIRA-mediated nucleosome assembly

Kang, B., Pu, M., Hu, G., Wen, W., Dong, Z., Zhao, K., Stillman, B., Zhang, Z. (2011) Phosphorylation of H4 Ser 47 promotes HIRA-mediated nucleosome assembly. Genes and Development, 25 (13). pp. 1359-1364. ISSN 08909369 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/21724829
DOI: 10.1101/gad.2055511

Abstract

Histone H3 variant H3.3, while differing from canonical H3 (H3.1) by only five amino acids, is assembled into nucleosomes, along with histone H4, at genic regions by the histone chaperone HIRA, whereas H3.1 is assembled into nucleosomes in a CAF-1-dependent reaction. Here, we show that phosphorylation of histone H4 Ser 47 (H4S47ph), catalyzed by the PAK2 kinase, promotes nucleosome assembly of H3.3-H4 and inhibits nucleosome assembly of H3.1-H4 by increasing the binding affinity of HIRA to H3.3-H4 and reducing association of CAF-1 with H3.1-H4. These results reveal a mechanism whereby H4S47ph distinctly regulates nucleosome assembly of H3.1 and H3.3. © 2011 by Cold Spring Harbor Laboratory Press.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > nucleosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression > phosphorylation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression
CSHL Authors:
Communities: CSHL labs > Stillman lab
CSHL Cancer Center Program > Gene Regulation and Cell Proliferation
Highlight: Stillman, Bruce W.
Depositing User: Matt Covey
Date Deposited: 05 Feb 2013 16:36
Last Modified: 20 Jun 2017 16:29
PMCID: PMC3134079
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27207

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