Khan, Z., Amini, S., Bloom, J. S., Ruse, C., Caudy, A. A., Kruglyak, L., Singh, M., Perlman, D. H., Tavazoie, S. (2011) Accurate proteome-wide protein quantification from high-resolution N-15 mass spectra. Genome Biology, 12 (12). ISSN 1474-7596
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Abstract
In quantitative mass spectrometry-based proteomics, the metabolic incorporation of a single source of N-15-labeled nitrogen has many advantages over using stable isotope-labeled amino acids. However, the lack of a robust computational framework for analyzing the resulting spectra has impeded wide use of this approach. We have addressed this challenge by introducing a new computational methodology for analyzing N-15 spectra in which quantification is integrated with identification. Application of this method to an Escherichia coli growth transition reveals significant improvement in quantification accuracy over previous methods.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | escherichia-coli quantitative proteomics stationary-phase gene-expression spectrometry identification conversion algorithm culture rates |
| Subjects: | bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification biotechnology > chromatography > protein purification |
| CSHL Authors: | |
| Communities: | CSHL labs > Pappin lab |
| Depositing User: | Matt Covey |
| Date: | 2011 |
| Date Deposited: | 06 Feb 2013 14:53 |
| Last Modified: | 06 Feb 2013 14:53 |
| PMCID: | PMC3334617 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/27195 |
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